Project description:The experiment was designed to test how tumour conditoned media from STm-delta-aroA infected tumour organoids would modulate T cell activation. Using splenocytes from Nr4a3-Tocky Great Smart-17A mice, T cells were activated with soluble anti-CD3 and anti-CD28 antibodies. At 4 and 16 h live CD4+ T cells were FACS sorted and RNA extracted. Analysis revealed that T cells exposed to STm-delta-aroA conditioned tumor medium failed to upregulate metabolic pathways associated with canonical T cell activation.
Project description:In this project we investigated the effect of YAP1 mutations on the interactome. We investigate the effect of S61A, S109A, S127A, S138A, S143A, S367A, 5SA, delta WW1, delta WW2, delta WW1/WW2
Project description:Arabidopsis transcription factor SHOOT MERISTEMLESS (STM) expression was induced using the dexamethasone-inducible TGV system in the Ler background and differentially expressed genes were identified compared to an induced empty-vector control line at each time point using RMA and LIMMA analysis.
Project description:The experiment was designed to test how autocrine TNF-alpha impacts human CD4+ T cell activation. Naïve CD4+ T cells from healthy human blood donors were either left unactivated or activated with anti-CD3/anti-CD28 in presence of an isotype control antibody or anti-TNF-alpha antibody. RNA was extracter at 48 hours. Analysis revealed that anti-TNF-alpha treatment prevented upregulation of activation-induced T cell metabolic, signaling and effector molecules.