Ontology highlight
ABSTRACT:
PROVIDER: PRJNA906658 | ENA |
REPOSITORIES: ENA
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Project description:Herbal compatibility is the knowledge of which herbs to combine in traditional Chinese medicine (TCM) formulations. The lack of understanding of herbal compatibility is one of the key problems for the application and popularization of TCM in western society. Because of the chemical complexity of herbal medicines, it is simpler to begin to conduct compatibility research based on herbs rather than component plant secondary metabolites. We have used transcriptome analysis to explore the effects and interactions of two plant extracts (Kushen and Baituling) combined in Compound Kushen Injection (CKI). Based on shared chemical compounds and in vitro cytotoxicity comparisons, we found that both the major compounds in CKI, and the cytotoxicity effects of CKI were mainly derived from the extract of Kushen (Sophorae flavescentis). We generated and analyzed transcriptome data from MDA-MB-231 cells treated with single-herb extracts or CKI and results showed that Kushen contributed to the perturbation of the majority of cytotoxicity/cancer related pathways in CKI such as cell cycle and DNA replication. We also found that Baituling (Heterosmilax yunnanensis Gagnep) could not only enhance the cytotoxic effects of Kushen in CKI, but also activate immune-related pathways. Our analyses predicted that IL-1[beta] gene expression was upregulated by Baituling in CKI and we confirmed that IL-1[beta] protein expression was increased using an ELISA assay. Altogether, these findings help to explain the rationale for combining Kushen and Baituling in CKI, and show that transcriptome analysis using single herb extracts is an effective method for understanding herbal compatibility in TCM.
2019-08-20 | GSE130358 | GEO
Project description:We wanted to measure the transcriptomic changes of P.aeruginosa (PAO1 strain) when treated with plant extracts (Melastoma dodecandrum Lour, and Potentilla indica) and antibiotics (triclosan, rifampicin, gentamicin and ciprofloxacin) against DMSO control to aid in understanding the mode of action of our plant extracts. PAO1 samples were treated with plant extracts and antibiotics at MIC, for 24h at 37°C with OD readings taken at 600 nm every 10 mins using a microplate reader.
2023-03-01 | E-MTAB-12652 | biostudies-arrayexpress
Project description:Small RNA-seq from FPLC-fractionated plant crude extracts
| PRJNA529572 | ENA
Project description:The effect of plant extracts on human macrophage activation
| PRJNA388477 | ENA
Project description:Bioremediation of persistent organic pollutants using plant liquid fermented extracts
| PRJNA1107565 | ENA
Project description:Graviola (Annona muricata) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic study to reveal new bioactivities of these leave extracts on both conditioned media and extracts of HT-1080 fibrosarcoma treated cells. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from An-nona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated. Additional experimental data reveal that these extracts protect from ferroptosis to both HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells.
2023-10-24 | PXD042354 | Pride