Project description:Nanoscale zero valent iron (nZVI) is used to remediate aquifers polluted by organochlorines or heavy metals has been also considered for elimination of harmful algal blooms. Highly reactive nZVI then affects microorganisms in the application area. To date, various nZVI toxicity endpoints have been studied on different organisms. However, the underlying mechanistic related to iron defense pathways have not been explained sufficiently. Here we aim to describe the physiological and transcriptomic response of the microalga, Raphidocelis subcapitata ATCC 22662, to 100 mg/L of non-reactive nFe3O4, and reactive nZVI. The combined effect of shading by nanoparticles and release of Fe2+ from nZVI posed a stronger inhibition leading to deformed cells and cytosol leakage in 15% of cells. Transcriptomic analysis confirmed the stronger physiological effect of nZVI (7,380 differentially expressed genes [DEGs]) than nFe3O4 (4,601 DEGs) after 1 h. nZVI (but not nFe3O4) caused increased DNA repair and replication, while deactivated carbohydrate-energy metabolisms, mitochondria signaling, and transmembrane ion transport. The defense response of algal cells was immediate to successfully face oxidative stress.
Project description:Green alga (Raphidocelis subcapitata) response to nanoscale zero valent iron (nZVI): The DNA damage repair pathways as sentinel response
Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..
Project description:This study aims at investigating the effects of methylmercury on a microalga using a transcriptomic approach. Algal cells were exposed two hours in a simplified artificial medium spiked with 0.05 or 0.5 nM MeHg. Total RNA was extracted using TRI Reagent®. Libraries were prepared with the Illumina TruSeq Stranded mRNA kit and sequenced on an Illumina HiSeq 2500 System.
Project description:We report the application of high-throughput analysis of genes related to anthocyanin synthesis in red and green walnuts based on transcriptome sequencing technology. By obtaining a sequence of more than 3 billion bases from mRNA, we found genes that are differentially expressed with anthocyanin synthesis in 4 leaf growth stages and 3 peel growth stages of red and green walnuts.
