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Engineering an inhibitor resistant human CSF1R variant for microglia replacement

ABSTRACT: Engineering an inhibitor resistant human CSF1R variant for microglia replacement

PROVIDER: PRJNA909236 | ENA |

REPOSITORIES: ENA

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Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVivo_RNA]

Project description:Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVivo_RNA]

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Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVitro_RNA]

Project description:Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVitro_RNA]

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Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVitro_RNA]

Project description:iPSC-derived microglia expressing the wildtype, G795A, or G795C CSF1R variants were generated in vitro and treated with either DMSO or Plexxicon 5622 in order to compare the gene expression differences exising between genotypes at baseline or following CSF1R inhibtion.

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Engineering an inhibitor resistant human CSF1R variant for microglia replacement [InVivo_RNA]

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Engineering an inhibitor resistant human CSF1R variant for microglia replacement

Project description:This SuperSeries is composed of the SubSeries listed below.

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Efficient strategies for microglia replacement in the central nervous system

Project description:Microglia are important immune cells in the central nervous system (CNS). Dysfunctions of gene-deficient microglia contribute to the development and progression of multiple CNS diseases. Microglia replacement by nonself cells has been proposed to treat microglia associated disorders. However, most of attempts are failed due to low replacement efficiencies, such as with the traditional bone marrow transplantation approach. In this study, we develop three efficient strategies for microglia replacement: microglia replacement by bone marrow transplantation (mrBMT), microglia replacement by peripheral blood (mrPB) and microglia replacement by microglia transplantation (mrMT). mrBMT and mrPB allow microglia-like cells to efficiently replace resident microglia in the whole CNS. On the other hand, mrMT achieves microglia replacement in brain regions of interest. In summary, the present study offers effective tactics for microglia replacement with diverse application scenarios, which potentially opens up a window on treating microglia-associated CNS disorders.

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Project description:Purpose: Identify zebrafish control and csf1r-mutant microglia transcriptomes Methods: RNA sequencing was performed on FACS-sorted control microglia (3x), csf1ra-/- microglia (3x) and csf1ra-/-;b+/- microglia (3x). 10-20 million reads per sample were obtained. Reads were mapped to zebrafish genome GRC10. Results: We identified that csf1ra-/- or csf1ra-/-;b+/- microglia transcriptomes retain most of the microglia gene expression signature but mostly show changes in chemoklines expression.

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single-cell RNA-seq profile of microglia under acute Csf1r inhibition and early repopulation in adult brain

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