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Transcriptome profiling of EPB41L5 WT and KO podocytes

ABSTRACT: Transcriptome profiling of EPB41L5 WT and KO podocytes

PROVIDER: PRJNA909596 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22560443_1.fastq.gz	Fastqsanger.gz
	SRR22560443_2.fastq.gz	Fastqsanger.gz
	SRR22560444_1.fastq.gz	Fastqsanger.gz
	SRR22560444_2.fastq.gz	Fastqsanger.gz
	SRR22560445_1.fastq.gz	Fastqsanger.gz

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Project description:We generated NUP133 WT and KO podocytes to model human hereditary SRNS in vitro. A subtype of SRNS is caused by monogenetic mutations of the nuclear pore complex including NUP133. Immortalized human podocytes were genome edited applying the CRISPR/Cas9 technique and two independent NUP133 sgRNAs. Two monoclonal cell backgrounds were used to create two matching sets of NUP133 WT and KO cells (WT-1 versus KO-1 and WT-2 versus KO-2). Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis was performed in duplicate for each cell line. NUP133 KO podocytes exhibit vast transcriptional changes compared to WT cells.

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Transcriptome profiling of EPB41L5 WT and KO podocytes

Project description:We generated EPB41L5 WT and KO podocytes to model human glomerular kidney disease in vitro. Immortalized human podocytes were genome edited applying the CRISPR/Cas9 technique. Two monoclonal cell lines were generated for WT and KO genotypes (WT-1, WT-2, KO-1 & KO-2). Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis was performed in duplicate for each cell line. EPB41L5 KO podocytes exhibit transcriptional changes compared to WT cells. Analyzed cell were previously described: Schell C, Rogg M, Suhm M, et al. The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier. Proc Natl Acad Sci U S A. 2017;114(23):E4621-E4630. doi:10.1073/pnas.1617004114

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