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Single-cell RNA-seq analysis of p53 knockout primary adult mouse astrocytes in vitro.

ABSTRACT: Single-cell RNA-seq analysis of p53 knockout primary adult mouse astrocytes in vitro.

PROVIDER: PRJNA909830 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22570649_1.fastq.gz	Fastqsanger.gz
	SRR22570649_2.fastq.gz	Fastqsanger.gz

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Single-cell RNA-seq analysis of p53 knockout primary adult mouse astrocytes in vitro.

Project description:To investigate the mechansims that underlie astrocyte dedifferentiation, we performed single cell RNA sequencing analysis of primary astrocytes isolated from adult mouse cortex after p53 loss and exposure to mitogens, EGF and and FGF. Primary astrocytes were isolated from cortices of 2-3month old inducible p53 knockout mice (GFAP-CreERT2;p53flox/flox;LSL-tdTomato) using Miltenyi Adult Brain Dissociation kit and ACSA-2 beads. Treatment with 4-hydroxytamoxifen (4OHT) induces p53 loss and tdTomato labelling in GFAP+ astrocytes. Astrocytes were treated with 4OHT in media supplemented with EGF and FGF to induce recombination and astrocyte dedifferentiation in vitro.

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