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Gene expression profile at single cell level of podocyte-specific I-PpoI expressing mice kidney cortex

ABSTRACT: Gene expression profile at single cell level of podocyte-specific I-PpoI expressing mice kidney cortex

PROVIDER: PRJNA909834 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22570937_1.fastq.gz	Fastqsanger.gz
	SRR22570937_2.fastq.gz	Fastqsanger.gz
	SRR22570938_1.fastq.gz	Fastqsanger.gz
	SRR22570938_2.fastq.gz	Fastqsanger.gz
	SRR22570939_1.fastq.gz	Fastqsanger.gz

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Gene expression profile at single cell level of podocyte-specific I-PpoI expressing mice kidney cortex

Project description:To investigate the alterations in cell populations in the kidney after podocyte DNA DSBs, we performed single-cell RNA sequencing (scRNA-seq) on the renal cortex of 12-week-old podocyte-specific I-PpoI expressing mice that had not yet developed glomerulosclerosis or fibrosis.

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Gene expression profile at single cell level of proximal tubular epithelial cell specific I-PpoI expressing mice kidney cortex

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Gene expression profile at single cell level of proximal tubular epithelial cell specific I-PpoI expressing mice kidney cortex

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Expression data for OVE26 diabetic mouse podocyte

Project description:Podocytes form filtration barrier through foot process around glomerualar basement membrane and selectively permit permeability of molecular smaller than albumin. Diabetes can cause podocyte pathological changes leading to high urine albumin level. Diabetic mouse model OVE26 has extremly high urine albumin and previously studies indicated its podocyte damaged. Here we try to find the key genes change in OVE26 diabetic mouse model podocyte by microarray assay while normal FVB mouse podocyte set as control. Podocyte eGFP transgenic mice were made on FVB background and crossbred to OVE26 diabetic model. Glomeruli isolated from OVE-GFP mice were digested by trypsin into signal cell. Podocytes with GFP were sorting out by FACS.

2012-03-02 | E-GEOD-36209 | biostudies-arrayexpress

Expression data for OVE26 diabetic mouse podocyte

2012-03-02 | GSE36209 | GEO

To investigate how the glycosylation of podocyte proteins changes during diabetic kidney disease

Project description:Podocytes are cells of the visceral epithelium in the kidneys and form a crucial component of the glomerular filtration barrier, contributing to size selectivity and maintaining a massive filtration surface. We are interested in pursuing a microarray analysis to identify the glycosylation-related genes that are modulated in podocytes during insulin and glucose stimulation. As such, we propose to isolate RNA from cultured AB 8/13 human podocyte cell line in their normal state and treated with different concentrations of insulin and glucose. Initially, we would like to analyze 12 samples (Normal control, 2 Starved controls, Insulin experiments 1-4, and glucose experiments 1-5). These experiments would give us information on the gene regulation changes in different healthy and diabetic conditions. In addition to providing possible identification of the unknown proteins involved with diabetic nephropathy, such analysis would provide novel information about early responses by podocytes in response to insulin and glucose level changes in vitro. The results from this analysis will be utilized to focus our research with regard to glycosylation of the proteins in the podocyte slit diaphragm.

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