Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of SNHG15-associated microRNAs in SNHG15 overexpression BT549 cells using miRNA-Seq

ABSTRACT: Identification of SNHG15-associated microRNAs in SNHG15 overexpression BT549 cells using miRNA-Seq

PROVIDER: PRJNA910073 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of SNHG15 Binding Protein in SNHG15 overexpression BT549 cells Using MS2 Pull down and Mass Spectrometry

Project description:SNHG15 is an oncogenic lncRNA in a variety of cancers including breast cancer, lung cancer, and hepatocellular carcinoma. In the present study, We used MS2 pulldown and MS spectrometry to Identification of SNHG15 Binding Protein in SNHG15 overexpression BT549 cells.

2022-12-27 | PXD039100 |

Identification of SNHG15-associated microRNAs in SNHG15 overexpression BT549 cells using miRNA-Seq

Project description:LncRNAs have been shown to act as molecular sponges for miRNAs, and hence functionally affect the activity of other RNA transcripts targeted by the sponged miRNAs. SNHG15 is an oncogenic lncRNA in a variety of cancers including breast cancer, lung cancer, and hepatocellular carcinoma.

2023-12-01 | GSE220496 | GEO

Transcriptome analysis of BT549 cells overexpressing SNHG15

Project description:LncRNA SNHG15 is an oncogenic lncRNA in a variety of cancers including breast cancer, lung cancer, and hepatocellular carcinoma. To screen genes regulated by SNHG15, we performed RNA-seq with stable SNHG15-overexpression BT549 cells, using BT549-pcIP2 as control.

2023-06-08 | GSE220492 | GEO

Identification of lncRNA UCA1-associated microRNAs in breast cancer cell line MDA231 using miRNA-Seq

Project description:LncRNA urothelial carcinoma-associated 1 (UCA1) is an oncogene in breast cancer. Previous reports indicates that lncRNAs may function as competing endogenous RNAs (ceRNAs) to sponge miRNAs, thereby modulating the derepression of miRNA targets. To characterize microRNAs that associated with UCA1, the microRNAs associated with UCA1 were extracted from the UCA1-MS2 RNP complexes and analyzed by miRNA-seq.

2016-12-14 | GSE92319 | GEO

miRNA overexpression modulates neutrophil migration

Project description:miRNA overexpression modulates neutrophil migration

| PRJNA704949 | ENA

Coordinated regulation of polycomb group complexes through microRNAs in cancer

Project description:To determine the altered mRNA expression signatures upon overexpressing EZH2 regulated microRNAs in cancer. To identify EZH2 regulated microRNAs in cancer. Two-condition experiment: Each EZH2 microRNA was overexpressed and compared to a control RNA in BT549 breast cancer cells. In DU145 prostate cancer cell EZH2 was knocked down and comapred to EZH2 scrambled siRNA. Other cell lines were monitored for miRNA expression as they are known to have high endogenous levels of EZH2.

2011-10-20 | E-GEOD-26996 | biostudies-arrayexpress

BT549 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA

2012-09-02 | GSE31425 | GEO

Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1

Project description:The 22Rv1 and PC-3 cells were transduced with shMIMIC human lentiviral vectors (Horizon Discovery, Cambridge, UK) to stably overexpress miRNA-23c or -4328. The SMARTvector Non-Targeting Control (NTC) was expressed to serve as a negative control. The vectors contained a turbo green fluorescent protein (turboGFP) and a puromycin resistance gene cassette. After transduction, cells were cultured in a medium supplemented with 5 µg/mL puromycin (Takara Bio, Tokyo, Japan) for antibiotic selection. Overexpression was confirmed by monitoring the turboGFP by fluorescence microscopy and by RT-qPCR analysis of miRNA-23c and -4328 levels. Relative protein quantification was performed to compare protein expression in 22Rv1 and PC-3 single cell clones overexpressing miRNA-23c and -4328, compared to corresponding NTC cells. Single cell clones overexpressing either miRNA-23c (n = 3), -4328 (n = 3) or NTC (n = 3) were analyzed in triplicate.

2023-04-24 | PXD041683 | Pride

miR-200c induction in BT549 TNBC cells

Project description:We performed microarray analysis on BT549 cells that express miR-200c in a doxycycline (dox)-inducible manner from the miR-200c-TripZ system.

2017-12-20 | GSE108271 | GEO

Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

Project description:Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

| PRJNA524346 | ENA