Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

ABSTRACT: Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

PROVIDER: PRJNA911927 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22709961.fastq.gz	Fastqsanger.gz
	SRR22709962.fastq.gz	Fastqsanger.gz
	SRR22709963.fastq.gz	Fastqsanger.gz
	SRR22709964.fastq.gz	Fastqsanger.gz
	SRR22709965.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

Project description:The airway epithelial cell line, 16HBE14o-, is an important cell model for studying airway disease. 16HBE14o- cells were originally generated from primary human bronchial epithelial cells by SV40-mediated immortalization, a process that is associated with genomic instability through long-term culture. Here we explore the heterogeneity of these cells, with respect to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) transcript and protein. We isolate clones of 16HBE14o- with stably higher and lower levels of CFTR in comparison to bulk 16HBE14o-, designated CFTRhigh and CFTRlow. Detailed characterization of the CFTR locus in these clones by ATAC-seq and 4C-seq showed open chromatin profiles and higher order chromatin structure that correlate with CFTR expression levels. . Transcriptomic profiling of CFTRhigh and CFTRlow cells showed that the CFTRhigh cells had an elevated inflammatory/ innate immune response phenotype. These results encourage caution in interpreting functional data from clonal lines of 16HBE14o- cells, generated after genomic or other manipulations.

2023-06-07 | GSE220852 | GEO

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.

Project description:Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.

| PRJNA911760 | ENA

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [RNA-seq]

2023-06-07 | GSE220854 | GEO

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [ATAC-seq]

2023-06-07 | GSE220853 | GEO

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [ATAC-seq]

Project description:Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [ATAC-seq]

| PRJNA911926 | ENA

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [RNA-seq]

Project description:Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [RNA-seq]

| PRJNA911925 | ENA

Phosphoproteome of airway epithelial cell in response to infection with Staphylococcus aureus

Project description:Here we used an unbiased proteomic and phosphoproteomic approach to characterize the response of 16HBE14o- airway epithelial cells following infection with S. aureus.

2016-12-14 | PXD002776 | Pride

Single cell multiomic readouts reveal that cellular heterogeneity impacts lipid nanoparticle-mediated mRNA delivery

Project description:Single cell multiomic readouts reveal that cellular heterogeneity impacts lipid nanoparticle-mediated mRNA delivery

| PRJNA773730 | ENA

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-07 | GSE220855 | GEO

Shigella flexneri 4c strain:4c

Project description:Shigella flexneri 4c strain:4c Genome sequencing and assembly

| PRJNA356350 | ENA