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Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

ABSTRACT: Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

PROVIDER: PRJNA911927 | ENA |

REPOSITORIES: ENA

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Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]

Project description:The airway epithelial cell line, 16HBE14o-, is an important cell model for studying airway disease. 16HBE14o- cells were originally generated from primary human bronchial epithelial cells by SV40-mediated immortalization, a process that is associated with genomic instability through long-term culture. Here we explore the heterogeneity of these cells, with respect to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) transcript and protein. We isolate clones of 16HBE14o- with stably higher and lower levels of CFTR in comparison to bulk 16HBE14o-, designated CFTRhigh and CFTRlow. Detailed characterization of the CFTR locus in these clones by ATAC-seq and 4C-seq showed open chromatin profiles and higher order chromatin structure that correlate with CFTR expression levels. . Transcriptomic profiling of CFTRhigh and CFTRlow cells showed that the CFTRhigh cells had an elevated inflammatory/ innate immune response phenotype. These results encourage caution in interpreting functional data from clonal lines of 16HBE14o- cells, generated after genomic or other manipulations.

2023-06-07 | GSE220852 | GEO

Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.

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Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [ATAC-seq]

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Phosphoproteome of airway epithelial cell in response to infection with Staphylococcus aureus

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Project description:This SuperSeries is composed of the SubSeries listed below.

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