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Characterization of DkaiC mutants of Pseudomonas putida KT2440/2 and Pseudomonas protegens CHA0

ABSTRACT: Characterization of DkaiC mutants of Pseudomonas putida KT2440/2 and Pseudomonas protegens CHA0

PROVIDER: PRJNA912661 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22750601_1.fastq.gz	Fastqsanger.gz
	SRR22750601_2.fastq.gz	Fastqsanger.gz
	SRR22750602_1.fastq.gz	Fastqsanger.gz
	SRR22750602_2.fastq.gz	Fastqsanger.gz
	SRR22750603_1.fastq.gz	Fastqsanger.gz

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Characterization of DkaiC mutants of Pseudomonas putida KT2440/2 and Pseudomonas protegens CHA0

Project description:KaiC is the central cog of the circadian clock in Cyanobacteria. Close homologs of this protein are widespread among bacteria not known to have a circadian physiology. The function, interaction network, and mechanism of action of these KaiC homologs are still largely unknown. Here, we focus on KaiC homologs found in environmental Pseudomonas species. We characterize experimentally the only KaiC homolog present in Pseudomonas putida KT2440 and Pseudomonas protegens CHA0. Through phenotypic assays and transcriptomics, we show that KaiC is involved in osmotic and oxidative stress resistance in P. putida and in biofilm production in both P. putida and P. protegens.

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