Project description:Podosphaera xanthii is the main causal agent of powdery mildew (PM) disease for Cucurbita pepo. Disease control is attained principally by applications of chemical fungicides, along with parallel use of tolerant crop varieties and alternate application of elicitors to control development of disease resistance. To get insight into C. pepo molecular responses to P. xanthii infection and elicitor treatment we studied the proteomic profile differences at the phyllosphere of a zucchini cultivar susceptible to PM, at the onset of P. xanthii (PX) infection and after application of Reynoutria sachalinensis (RS) plant extract, respectively, using a nano-LC-HRMS/MS, Q-Exactive-Orbitrap approach. Analysis of peptide sequences regarding four treatment groups (Control; PX; RS; and RSPX (PX-infected priorly treated with RS)) resulted in 2070 CuGenDB annotations. Three comparisons (treatments vs Control) encompassed most of the Differentially Expressed Proteins (DEPs). In these three comparisons, KEGG and Gene Ontology functional analyses highlighted unique differentially enriched pathways -some of which including highly expressed proteins- in PX-related (Proteasome, Pentose phosphate pathway, and Carbon fixation), RS-related (Biosynthesis of Secondary metabolites, Flavonoids, and Starch and Sucrose metabolism), and RSPX-related (Pyruvate metabolism and Polycomb repressive complex) comparisons respectively, suggesting distinct mechanisms of early plant responses modulated by PX and RS. Furthermore, in four out of six comparisons the Thiamine metabolism pathway was found to be enriched, suggesting a pivotal role in PX-induced responses.

2024-11-07 | PXD057221 | Pride

Podosphaera aphanis strain:C1

Project description:Podosphaera aphanis strain:C1 Genome sequencing and assembly

| PRJNA439380 | ENA

Podosphaera fusca

Project description:Podosphaera fusca Genome sequencing and assembly

| PRJNA976089 | ENA