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BET activity plays an essential role in control of stem cell attributes in Xenopus

ABSTRACT: BET activity plays an essential role in control of stem cell attributes in Xenopus

PROVIDER: PRJNA913912 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22812500.fastq.gz	Fastqsanger.gz
	SRR22812501.fastq.gz	Fastqsanger.gz
	SRR22812502.fastq.gz	Fastqsanger.gz
	SRR22812503.fastq.gz	Fastqsanger.gz
	SRR22812504.fastq.gz	Fastqsanger.gz

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BET activity plays an essential role in control of stem cell attributes in Xenopus

Project description:Here we examine the role that epigenetic readers of the BET (Bromodomain and Extra Terminal) family play in controlling the potential of pluripotent blastula and neural crest cells. We find that inhibiting BET activity leads to loss of pluripotency and inability to respond to lineage instructing cues at blastula stages, and a loss of neural crest at neurula stages.

2024-06-19 | GSE221330 | GEO

BET activity plays an essential role in control of stem cell attributes in Xenopus.

Project description:Neural crest cells are a stem cell population unique to vertebrate embryos that retains broad multi-germ layer developmental potential through neurulation. Much remains to be learned about the genetic and epigenetic mechanisms that control the potency of neural crest cells. Here, we examine the role that epigenetic readers of the BET (bromodomain and extra terminal) family play in controlling the potential of pluripotent blastula and neural crest cells. We find that inhibiting BET activity leads to loss of pluripotency at blastula stages and a loss of neural crest at neurula stages. We compare the effects of HDAC (an eraser of acetylation marks) and BET (a reader of acetylation) inhibition and find that they lead to similar cellular outcomes through distinct effects on the transcriptome. Interestingly, loss of BET activity in cells undergoing lineage restriction is coupled to increased expression of genes linked to pluripotency and prolongs the competence of initially pluripotent cells to transit to a neural progenitor state. Together these findings advance our understanding of the epigenetic control of pluripotency and the formation of the vertebrate neural crest.

| S-EPMC11266789 | biostudies-literature

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Epithelial EP4 plays an essential role in maintaining homeostasis in colon

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TOX2 regulates human natural killer cell development by controlling T-BET expression

Project description:Thymocyte selection-associated high mobility group box protein family member 2 (TOX2) is a transcription factor belonging to the TOX family that shares a highly conserved high mobility group DNA binding domain with the other TOX members. While TOX1 has been shown to be an essential regulator of T-cell and natural killer (NK) cell differentiation in mice, little is known about the roles of the other TOX family members in lymphocyte development, particularly in humans. In this study, we found that TOX2 was preferentially expressed in mature human NK cells and was upregulated during in vitro differentiation of NK cells from human umbilical cord blood (UCB)M-bM-^@M-^Sderived CD34+ cells. Gene silencing of TOX2 intrinsically hindered the transition between early developmental stages of NK cells, while overexpression of TOX2 enhanced the development of mature NK cells from UCB CD34+ cells. We subsequently found that TOX2 was independent of ETS-1 but could directly upregulate the transcription of TBX21 (encoding T-BET). Overexpression of T-BET rescued the TOX2 knockdown phenotypes. Given the essential function of T-BET in NK cell differentiation, TOX2 therefore plays a crucial role in controlling normal NK cell development by acting upstream of TBX21 transcriptional regulation. survey of NK cells over time

2014-12-22 | E-GEOD-58589 | biostudies-arrayexpress