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Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

ABSTRACT: Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

PROVIDER: PRJNA913972 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22821534_1.fastq.gz	Fastqsanger.gz
	SRR22821534_2.fastq.gz	Fastqsanger.gz
	SRR22821535_1.fastq.gz	Fastqsanger.gz
	SRR22821535_2.fastq.gz	Fastqsanger.gz
	SRR22821536_1.fastq.gz	Fastqsanger.gz

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Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

Project description:We compared the transcriptomic changes in cell populations of the arcuate nucleus of the hypothalamus in 21 day treated DOCA-salt male mice versus sham male mice using high-throughput single-nucleus RNA-seq.

2023-03-20 | GSE221367 | GEO

Single-nucleus transcriptome sequencing reveals a specific neuronal subtype in the arcuate nucleus.

Project description:Over recent decades, research has established the hypothalamus, particularly the arcuate nucleus, as a central hub for the homeostatic control of energy balance. The hypothalamic arcuate nucleus is a pivotal regulator of energy balance, containing anorexic proopiomelanocortin (POMC) neurons and orexigenic agouti-related peptide (AgRP) neurons, which exert opposing effects on energy homeostasis. However, the molecular identity of “non-AgRP, non-POMC” neurons remains unclear. We identified a distinct neuronal subtype characterized by the absence of Agrp and Pomc expressions, while exhibiting robust Crabp1 expression.

2024-12-10 | GSE276414 | GEO

Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus

Project description:We report RNA sequencing of single Agrp neurons isolated from the arcuate nucleus of the hypothalamus. Analyses of Agrp neuron transcriptomes reveals differential expression of receptors for multiple neuromodulators. Neuroanatomical mapping of known ligands of the receptors define subsets of neurons directly upstream of AgRP neurons in specific brain areas.

2023-03-27 | GSE227909 | GEO

Transcriptomic response of the arcuate nucleus to oral corticosterone treatment

Project description:Glucocorticoids (GCs) are widely prescribed to treat inflammatory and autoimmune conditions. However, patients often develop adverse metabolic effects, including hyperphagia, obesity, and hyperglycemia.The arcuate nucleus has an established role in the central control of energy balance. In this study we examined the transcriptomic response of the arcuate nucleus to oral corticosterone treatment. Gene expression changes were measured after 2 days and 4 weeks of Cort treatment in ad libitum fed C57BL6/J mice.

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Estrogen Receptor alpha DNA-binding in the arcuate and AVPV nuclei of the hypothalamus in female mice

Project description:Coupling the release of pituitary hormones to the developmental stage of the oocyte is essential for female fertility. It requires estrogen to restrain kisspeptin (Kiss1) neuron pulsatility in the arcuate hypothalamic nucleus, while also exerting a surge-like effect on Kiss1 neuron activity in the AVPV hypothalamic nucleus. However, a mechanistic basis for this region-specific effect has remained elusive. Here, we provide functional insight into hypothalamic estrogen sensing by analyzing estrogen receptor alpha (ERα/ESR1) DNA binding events in the arcuate and AVPV nuclei of the hypothalamus in female mice.

2023-04-25 | GSE227540 | GEO

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence

2017-02-06 | GSE90806 | GEO

The transcriptomics of SLC18A3 positive cells from arcuate nucleus of rats in different pubertal development stages.

Project description:Based on our spatial transcriptomics sequencing data, we observed solute carrier family 18 member A3 (SLC18A3) was specificially expressed in kisspeptin-expressed neurons in arcuate nucleus (ARC). SLC18A3 as a surface marker was used to examine the isolation of living kisspeptin-expressed neurons by flow cytometric (FACS) assay. Fresh isolated ARC nucleus tissues from female Sprague-Dawley rats in postnatal day (PND)-25, 35 and 45 (n=10 each group) were harvested, and processed single-cell suspension for FACS assay by SLC18A3. Here, we obtained two populations according to the cell size by FSC/SSC signals, and further harvested a small proportion of Slc18a3+ cells from hypothalamus of PND-25, 35 and 45. Smart-seq was conducted to detect the transcriptome of four population namely large Slc18a3+/- cells as well as small Slc18a3+/- cells.

2021-10-04 | E-MTAB-10975 | biostudies-arrayexpress

A Molecular Census of Arcuate Hypothalamus and Median Eminence Cell Types

Project description:Drop-seq and single cell sequencing of mouse arcuate nucleus and median eminence. Please see below link for searchable cluster-based gene expression.

2017-01-31 | GSE93374 | GEO

Effect of early-life plane of nutrition on the transcriptional profile of the hypothalamic arcuate nucleus region in heifer calves

Project description:The aim of this study was to examine alterations in the transcriptional profile of the arcuate nucleus region of the hypothalamus between heifer calves offered varying planes of nutrition during early life. Angus X Holstein-Friesian heifer calves (19 ± 5 days of age, BW: 51.2 ± 7.8 kg, mean ± SD) were offered a high (HP, n=14) or moderate plane of nutrition (MP, n=15) from 3 to 21 weeks of age to achieve a target growth rate of 1.2 kg/d and 0.5 kg/d, respectively. At 21 weeks of age, all calves were euthanized and the arcuate nucleus region of the hypothalamus of each calf recovered. RNA was extracted transcriptional profiles examined using RNAseq. Compared with calves on the moderate plane of nutrition, a High plane of nutrition altered the expression of 80 genes within the arucate nucleus. Functional analysis of differentially expressed genes revealed functions including organismal development, behaviour, endocrine system development and function, lipid metabolism, molecular transport, small molecule biochemistry, cellular development, cellular growth and proliferation, and nervous system development and function to be affected by early life plane of nutrition in the arucate nucleus of heifer calves.

2021-07-14 | GSE153498 | GEO

Transcript Profiling of Arcuate Nucleus in Response to Electroacupuncture on Rats

Project description:The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and non responders (NR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in arcuate nucleus induced by 2Hz/100Hz electroacupuncture in HR and NR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in arcuate nucleus. Rats were exposed to different frequencies (2Hz or 100Hz) electroacupuncture stimulation for 30 min and nociceptive testing and returned to home cages for 1 hour before sacrificed. According to the sensitivity of the analgesic response to 2Hz or 100Hz EA stimulation, the rats divided into four groups: 2Hz-HR group, 2Hz-NR group, 100Hz-HR group and 100Hz-NR group. Subsequently analyzed their dorsal horn transcript profile using cDNA microarrays, the rats were without receiving electroacupuncture as control. The tissue of arcuate nucleus (Arc) of three rats was selected for transcript analysis in each group. The three control rats were mixed and labeled with cy5, each rat of experiment groups was labeled with cy3.

2011-07-07 | E-GEOD-30365 | biostudies-arrayexpress

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