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Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

ABSTRACT: We compared the transcriptomic changes in cell populations of the arcuate nucleus of the hypothalamus in 21 day treated DOCA-salt male mice versus sham male mice using high-throughput single-nucleus RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE221367 | GEO | 2023/03/20

REPOSITORIES: GEO

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Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

Project description:Single-nucleus RNA-seq of mouse arcuate nucleus of the hypothalamus after DOCA-salt treatment

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Project description:Based on our spatial transcriptomics sequencing data, we observed solute carrier family 18 member A3 (SLC18A3) was specificially expressed in kisspeptin-expressed neurons in arcuate nucleus (ARC). SLC18A3 as a surface marker was used to examine the isolation of living kisspeptin-expressed neurons by flow cytometric (FACS) assay. Fresh isolated ARC nucleus tissues from female Sprague-Dawley rats in postnatal day (PND)-25, 35 and 45 (n=10 each group) were harvested, and processed single-cell suspension for FACS assay by SLC18A3. Here, we obtained two populations according to the cell size by FSC/SSC signals, and further harvested a small proportion of Slc18a3+ cells from hypothalamus of PND-25, 35 and 45. Smart-seq was conducted to detect the transcriptome of four population namely large Slc18a3+/- cells as well as small Slc18a3+/- cells.

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