Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: scRNA-seq of human osteoclast precursors

PROVIDER: PRJNA914118 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22830948_1.fastq.gz	Fastqsanger.gz
	SRR22830948_2.fastq.gz	Fastqsanger.gz
	SRR22830949_1.fastq.gz	Fastqsanger.gz
	SRR22830949_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Identification of a novel role for FoxO3 isoform2 in osteoclastic inhibition

Project description:We performed RNA-seq experiments, one with the WT control and Foxo3KO osteoclast precursors after RANKL stimulation to identify genes regulated by Foxo3 in osteoclast precursors.

2019-09-25 | GSE135479 | GEO

90a46c29-4ef0-43b5-9aa4-5f4ae95e8535 - samples

Project description:Human data for transcriptome (scRNA-Seq) in CD34+ B cell precursors.

| EGAD00001010907 | EGA

Bone protection by inhibition of microRNA-182

Project description:We performed RNA-seq experiments, one with the WT control and Mir182ΔM/ΔM osteoclast precursors at baseline and after RANKL stimulation, the other with miR-182 gain of function approach using the control and Mir182mTg BMMs, to identify genes regulated by miR-182 in osteoclast precursors.

2018-09-04 | GSE108721 | GEO

Characterization of embryonic osteoclast precursors

Project description:Characterization of embryonic osteoclast precursors

| PRJNA515201 | ENA

Stepwise cell fate decision pathways during osteoclastogenesis at single-cell resolution

Project description:To unveil cell fate decision pathways during osteoclast differentiation at single-cell resolution, we performed single-cell RNA sequencing (scRNA-seq) on the osteoclast culture system, in parallel with bulk RNA-seq and proteome analyses

2020-10-06 | GSE147174 | GEO

Expression data from CD115+ preosteoclasts stimulated by conditioned medium from CT-26 cells

Project description:In osteolytic tumor, bone resportion can be occured after bone metastasis. Bone metastasis of colorectal cancer can induce obvious bone destruction. Aberrant activation of osteoclasts and the precursors is responsible for tumor-induced osteolysis. The biological process of osteoclast precursors could be modified by different tumor microenvironment. Microarrays were used to detail the differentially expressed genes or non-coding RNAs between normal osteoclast precursors and osteoclast precursors treated by secreta from CT-26 cells.

2021-08-14 | GSE182097 | GEO

Characterization of embryonic osteoclast precursors

Project description:Embryonically established osteoclast precursors expressing tdTomato reporter gene were analyzed by single cell RNA-sequencing. Data showed that hematopoietic stem cell independent yolk-sac erythromyeloid progenitors produced embryonic osteoclast precursors.

2019-01-16 | GSE125088 | GEO

Ga13 functions as a cytoskeletal and mitochondrial regulator to restrain osteoclast function

Project description:We performed RNA-seq experiments, one with the WT control and Ga13ΔM/ΔM osteoclast precursors at baseline and after RANKL stimulation.

2019-03-20 | GSE116550 | GEO

Transcription profiling of mouse osteoclastogenesis

Project description:Expressions of mRNA in osteoclast precursors treated with RANKL and M-CSF for 0, 24, and 72 h. We used DNA microarray to analyze the changes in mRNA expressions during osteoclastogegesis. Experiment Overall Design: Osteoclast precursors were induced from mouse bone marrow cells by M-CSF for 3 days and treated with RANKL. WE extracted total RNA and analyzed by DNA microarray.

2009-09-05 | E-GEOD-17563 | biostudies-arrayexpress

M-CSF priming of osteoclast precursors can cause osteoclastogenesis-insensitivity, which can be prevented and overcome on bone

Project description:Comparison of gene expression of the osteoclast precursor myeloid blast seeded on plastic and on bone, primed with M-CSF for 4 days and culture with M-CSF and RANKL for 1 day. Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bone marrow precursor myeloid blast was pre-cultured with M-CSF on plastic and on bone. M-CSF priming prior to stimulation with M-CSF and osteoclast differentiation factor RANKL resulted in a complete loss of osteoclastogenic potential without bone. This coincided with a steeply decreased expression of osteoclast genes TRACP and DC-STAMP, but an increased expression of the macrophage markers F4/80 and CD11b. Compellingly, M-CSF priming on bone accelerated the osteoclastogenic potential: M-CSF primed cells that had received only one day M-CSF and RANKL and were grown on bone already expressed an array of genes that are associated with osteoclast differentiation and these cells differentiated into osteoclasts within 2 days. This implies that adhesion to bone dictates the fate of osteoclast precursors. Common macrophage-osteoclast precursors may become insensitive to differentiate into osteoclasts and regain osteoclastogenesis when bound to bone or when in the vicinity of bone. Two conditions: Osteoclast precursors on plastic and on bone, n=4, dye swap

2014-06-02 | E-GEOD-58146 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data