Ontology highlight
ABSTRACT:
PROVIDER: PRJNA914498 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR22839329_1.fastq.gz | Fastqsanger.gz | |||
| SRR22839329_2.fastq.gz | Fastqsanger.gz | |||
| SRR22839330_1.fastq.gz | Fastqsanger.gz | |||
| SRR22839330_2.fastq.gz | Fastqsanger.gz | |||
| SRR22839331_1.fastq.gz | Fastqsanger.gz |
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Project description:Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model
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Project description:There is much evidence suggesting that gene-environment interactions underlying the developmental origins of the metabolic syndrome. Previously, we have induced a metabolic syndrome phenotype in mice though prenatal undernutrition; however, the C57BL/6J strain exhibited significantly more metrics of the metabolic syndrome than the A/J strain. We used microarrays to detail the global programme of gene expression underlying the strain differences in the prenatal induction of the adult development of the metabolic syndrome. We focused on liver function and gene expression because of it's centrol role in many aspects of the metabolic syndrome including glucose and lipid control. Keywords: disease state analysis between mouse strains Pregnant A/J and C57BL/6J mice were either exposed to prenatal undernutrition during pregnancy or control conditions. The livers from 32 male offspring (8 per group within each of the two strains) were dissected out at 7 months of age and total RNA was extracted and hybridization on Affymetrix microarrays.
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Project description:Ruminant livestock are one of the major contributors to carbon emission contributing the global warming issue. Methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen are known to differ between sheep with different digestive function and fermentation products such as metabolites. However, the molecular mechanism underpinning differences in methane emission remains to be fully elucidated. We extracted a membrane and cytosolic protein fraction of rumen epithelium proteins from both high (H) and low (L) CH4 emitting sheep. Protein abundance differences between the phenotypes were quantified using SWATH-mass spectrometry. We identified 92 proteins annotated as cell surface transporters, of which only solute carrier family (SLC) 40A1 had a greater fold change of protein expression in the high methane emission phenotype. The main difference in protein abundance we found were related to the metabolism of glucose, lactate and processes of cell defence against microbes in the epithelium of sheep in each group. To best of our knowledge, this represents one of the most comprehensive proteomes of ovine rumen epithelium to date.
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