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Whole genome bisulfite sequencing comparing the DNA methylation level between monkey ICSI and SCNT blastocysts

ABSTRACT: Whole genome bisulfite sequencing comparing the DNA methylation level between monkey ICSI and SCNT blastocysts

PROVIDER: PRJNA915106 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22889545_1.fastq.gz	Fastqsanger.gz
	SRR22889545_2.fastq.gz	Fastqsanger.gz
	SRR22889546_1.fastq.gz	Fastqsanger.gz
	SRR22889546_2.fastq.gz	Fastqsanger.gz
	SRR22889547_1.fastq.gz	Fastqsanger.gz

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Whole genome bisulfite sequencing comparing the DNA methylation level between monkey ICSI and SCNT blastocysts

Project description:Somatic cell nuclear transfer (SCNT) has been used to clone cynomolgus monkeys (Macaca fascicularis), but the cloning of other non-human primate species remains to be achieved. On the other hand, our histological examination indicated severe calcification of the placenta of SCNT fetuses. Additionally, we found that some of the maternal-biased imprinting genes were aberrantly lost in the cloned embryos by multi-omics analysis. We thus developed a trophoblast replacement (TR) method, providing an ICSI-derived placenta for the SCNT fetus, to support the full-term development of the cloned embryos after implantation. By combining this TR method with treatment with epigenetic modification factors, we obtained a healthy somatic cell cloned rhesus monkey. Thus, TR represents a useful approach for rhesus monkey cloning.

2022-12-27 | GSE221636 | GEO

Next generation sequencing comparing the gene expression between ICSI and SCNT blastocysts of monkey [RNA-seq]

2022-12-27 | GSE221634 | GEO

Next generation sequencing of monkey ICSI, SCNT and TR placentas

2022-12-27 | GSE221638 | GEO

Next generation sequencing comparing the gene expression between ICSI and SCNT blastocysts of monkey [RNA-seq]

Project description:Next generation sequencing comparing the gene expression between ICSI and SCNT blastocysts of monkey [RNA-seq]

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Next generation sequencing of monkey ICSI, SCNT and TR placentas

Project description:Next generation sequencing of monkey ICSI, SCNT and TR placentas

| PRJNA915104 | ENA

Whole genome bisulfite sequencing of hybrid monkey fibroblast

2022-12-27 | GSE221637 | GEO

mRNA expression data from monkey PG-haESCs, ICSI-derived ESCs and somatic fibroblasts

Project description:Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes. Here,we report the derivation of haESCs from monkey parthenogenic blastocysts. These cells, which we designated PG-haESCs (parthenogenic haploid embryonic stem cells), express classical ESC markers, are pluripotent, and can differentiate to different cell lines from all three embryonic germ layers in vivo and in vitro. We used microarrays to compare the gene expression levels among PG-haESC, ICSI-derived ESCs and female monkey somatic fibroblasts. We used ICSI-derived ESCs and somatic fibroblasts isloated from female individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix Rhesus Macaque array.

2013-06-26 | E-GEOD-43978 | biostudies-arrayexpress

mRNA expression data from monkey PG-haESCs, ICSI-derived ESCs and somatic fibroblasts

2013-06-26 | GSE43978 | GEO

SCNT embryos are defective for H3K27me3 imprinting [Bisulfite-seq]

Project description:Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), yet the success rate remains very low. Recent studies have revealed two epigenetic barriers, H3K9me3 in donor cells and abnormal Xist activation, that impede SCNT reprogramming. Here we overcome both barriers by combining the use of Xist knockout donor cells and overexpressing Kdm4d, which allowed us to achieve the highest mouse cloning efficiency. However, SCNT-associated developmental defects and abnormal placenta were still observed, suggesting the existence of additional epigenetic defects in these SCNT embryos. Comparative DNA methylome analysis of IVF and SCNT blastocysts identified many abnormally methylated regions in SCNT embryos, despite successful global methylome reprogramming. Strikingly, allelic transcriptome analyses of SCNT blastocysts revealed a complete loss-of-imprinting at the H3K27me3-dependent imprinted genes, which may account for postimplantation developmental defects of SCNT embryos. This study thus not only provides the most efficient method for mouse cloning but also points the way for further improve SCNT cloning.

2018-06-27 | GSE112528 | GEO

Transcriptional profiling of rhesus monkey nuclear transfer embryonic stem cells

Project description:Derivation of embryonic stem cells (ESC) genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing any immunorejection issues. However, no primate nuclear transfer embryonic stem (ntES) cell lines have been derived to date. Here, we used a modified SCNT technique to produce rhesus macaque SCNT blastocysts at a relatively high efficiency from adult donor cells and we successfully derived two primate ntES cell lines from 304 oocytes (an overall efficiency of 0.7%). Nuclear and mitochondrial DNA analysis confirmed the ntES cell lines were derived from rhesus monkey SCNT blastocysts and both rhesus monkey ntES cell lines exhibited a normal ESC morphology, expressed key stemness markers, were transcriptionally indistinguishable from control ESC lines and differentiated into multiple cell types. This is, to our knowledge, the first confirmed derivation of primate ntES cell lines. Keywords: rhesus monkey somatic cell nuclear transfer embryonic stem cells

2007-08-29 | GSE7748 | GEO

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