Project description:The Pichia fermentans strain DISAABA 726 is a biofilm-forming yeast that has been proposed as biocontrol agent to control brown rot (Monilia spp.) on apple fruit. However, when inoculated on peach fruit, strain 726 shows yeast-like to pseudohyphal morphological transition coupled to a pathogenic behaviour. To identify the proteins potentially involved in the transition process, P. fermentans 726 proteome was analysed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry analysis.
Project description:Comparison of transcription profile of Pichia pastoris cells grown on Glucose medium with Pichia pastoris cells grown on Methanol/Glycerol medium, the fermentations were done in a chemostat.
Project description:Comparison of transcription profile of Pichia pastoris cells grown on Glucose medium with Pichia pastoris cells grown on Methanol/Glycerol medium, the fermentations were done in a chemostat. 2 color experiment in reference design. Pichia pastoris reference mix [mixed pool of Pichia pastoris cells sampled from various conditions including cells grown on glycerine, glucose and methanol, on full andminimal medium, in stationary and exponential growth phase, and in different stress states]