Project description:Inflorescence apices of hec1,2,3 triple mutants and ethanol induced 35S:AlcR, AlcA:HEC1 plants were copared to wild-type or ethanol induced 35S:AlcR, AlcA:GUS apices, respectively.
Project description:The Arabidopsis cytochrome P450 KLUH (KLU)/CYP78A5 promotes organ growth in a non-cell autonomous manner. To identify genes regulated by KLU activity, homozygous klu-2 mutants carrying constructs for EtOH-inducible overexpression of wild-type KLU (35S::AlcR-AlcA::KLU) or of enzymatically inactive KLU protein (35S::AlcR-AlcA::KLUmut) were induced with EtOH and sampled at 90 min and 240 min after induction for gene expression changes. Keywords: Time course, genetic modification
Project description:Inducible overexpression of STM by AlcR / AlcA system. Plants harboring 35S::AlcR/AlcA::GOI (GUS control, STM and STM-VP16)constructs were grown in continous light for 12 days and induced with 1% Ethanol. After 12h of EtOH treatment, seedlings were dissected and RNA was processed from the shoot apex and young leaves. Affymetrix Ath1 arrays were hybridized in duplicates from each experiment.
Project description:Inducible overexpression of Arabidopsis meristem regulators by AlcR / AlcA system. Plants harboring 35S::AlcR/AlcA::GOI (GUS control, LEAFY, SHOOTMERSTEMLESS, WUSCHEL)constructs were grown in continous light for 12 days and induced with 1% Ethanol. After 12h of EtOH treatment, seedlings were dissected and RNA was processed from the shoot apex and young leaves. Affymetrix Ath1 arrays were hybridized in duplicates from each experiment.
Project description:Transcriptional profiling of A. nidulans comparing the mutant strain alcApkcA ΔcnaA and ΔcnaA grown on alcA inducing conditions. The main objective was to identifiy genes related to ΔcnaA phenotype supression by pkcA overexpression. The experiment was further validated by real-time PCR.
Project description:Using transcriptomic analysis we show that transcription factor NapA is required for the regulation of at least 214 genes in intact asexual spores, including ethanol utilization genes alcR, alcA and aldA, and large groups of genes encoding proteins involved in transcriptional regulation, drug detoxification, carbohydrate utilization and secondary metabolism, comprising multiple oxidoreductases, membrane transporters and hydrolases.
