Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: MHV Passage in GS-441524 RNA Sequencing

PROVIDER: PRJNA922123 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR23024324_1.fastq.gz	Fastqsanger.gz
	SRR23024324_2.fastq.gz	Fastqsanger.gz
	SRR23024325_1.fastq.gz	Fastqsanger.gz
	SRR23024325_2.fastq.gz	Fastqsanger.gz
	SRR23024326_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68

Project description:Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and Kaposi’s sarcoma-associated herpesvirus (KSHV) and provides a small animal model to study the pathogenesis of gammaherpesvirus (γHV) infections. To completely explore the potential of the MHV-68 system for the investigation of gHV miRNAs, it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By using small RNA deep sequencing, we systematically investigated the MHV-68 miRNA expression profiles in both lytically and persistently infected cells. In addition to the known nine MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68 infected versus non-infected NIH3T3 fibroblasts and in TPA-treated versus non-treated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH3T3 cells, indicating a potential role of cellular miRNAs during MHV-68 infection. Our data will aid to fully explore the functions of gHV miRNAs.

2010-07-15 | GSE22938 | GEO

Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68

Project description:Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and KaposiM-bM-^@M-^Ys sarcoma-associated herpesvirus (KSHV) and provides a small animal model to study the pathogenesis of gammaherpesvirus (M-NM-3HV) infections. To completely explore the potential of the MHV-68 system for the investigation of gHV miRNAs, it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By using small RNA deep sequencing, we systematically investigated the MHV-68 miRNA expression profiles in both lytically and persistently infected cells. In addition to the known nine MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68 infected versus non-infected NIH3T3 fibroblasts and in TPA-treated versus non-treated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH3T3 cells, indicating a potential role of cellular miRNAs during MHV-68 infection. Our data will aid to fully explore the functions of gHV miRNAs. A mouse fibroblast cell line infected with/without MHV-68 and a MHV-68 infected mouse B lymphoma cell line treated with/without TPA (4 samples in total) were examined.

2010-07-15 | E-GEOD-22938 | biostudies-arrayexpress

dsRNA IP of Coronavirus Infected IFNAR-/- BMDMs

Project description:RNA-sequencing data of mock, wild-type, and EndoUmut MHV A59 infected IFNAR-/- bone-marrow derived macrophages. RNA was isolated at 6 hours post infection and immunoprecipitated with anti-dsRNA antibody to determine which RNA are forming a dsRNA epitope during viral infection. RNAs were mapped to C57Bl/6 Mouse genome or MHV-A59 genome (Genbank accession # AY910861).

2020-02-07 | GSE144886 | GEO

Project description:Direct RNA sequencing of MHV-WT

| PRJNA623578 | ENA

Project description:Direct RNA sequencing of MHV-ExoN(-)

| PRJNA623580 | ENA

Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection

Project description:The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs have 3’ poly(A) tails. The terminal addition of uridines to poly(A) tails has been shown to be a widespread modification. Here, we investigated the presence of 3' end additions on the MHV RNA poly(A) tails. To this end, we infected NCTC cells with MHV and isolated RNA at 24-hours post-infection (hpi). While the median poly(A) tail length of the MHV RNAs is around 50 nucleotides, we observed a peak of uridylation in transcripts with poly(A) tails about 40 nucleotides long

2023-03-23 | GSE200414 | GEO

Determination of MHV RNA poly(A) tail length and 3' end additions upon 17-CL1 cells infection [17-CL1_WT]

Project description:The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs are 3’ polyadenylated. The length of the 3’ poly(A) tail is known to change during infection, but little is known about the molecular mechanisms driving this change. Here, we investigate the presence of terminal uridylation and terminal guanylation on the MHV poly(A) tail during infection. We infected 17-CL1 cells with MHV and isolated RNA at 24- and 48-hour post-infection (hpi). We observed that MHV RNAs poly(A) tails shortened during infection and that short poly(A) tails of about 20 nucleotides are highly uridylated.

2023-03-23 | GSE200412 | GEO

Physiologic RNA Targets and Refined Sequence Specificity of Coronavirus EndoU

Project description:Coronavirus EndoU inhibits double-stranded RNA (dsRNA)-activated antiviral responses; however, it is unclear how this occurs because the physiologic RNA substrates of EndoU are unknown. In this study, we used Mouse Hepatitis Virus (MHV)-infected bone-marrow-derived macrophage (BMM) and cyclic phosphate cDNA sequencing to identify the RNA targets of EndoU

2020-09-18 | GSE147852 | GEO

Molecular Signature of CAID Syndrome: Noncanonical Roles of SGO1 in Regulation of TGF-β Signaling and Epigenomics. [RNA-seq]

Project description:RNA sequencing of human dermal fibroblasts from CAID patients passage 8 and passage 14

2018-10-31 | GSE110309 | GEO

microRNAs Restrain Proliferation in BRAFV600E Melanocytic Nevi [miRNA-Seq]

Project description:We conducted small RNA sequencing on low passage human melanocytes derived from melanocytic nevi and normal skin.

2021-05-13 | GSE174371 | GEO