Project description:Purpose: Establish a high-throughput method to transcriptionally define projection neurons, VECTORseq, that reimagines transgenes expressed by widely used retrogradely infecting viruses as multiplexed RNA barcodes that are detected in single-cell sequencing. Methods: mRNA profiles of adult mouse brains Conclusions: Retrograde viruses express mRNA at levels detectable in single-cell sequencing. Different transgenes can be multiplexed in a single sequencing run. VECTORseq identifies both cortical and subcortical projection neurons. VECTORseq defined new superior colliculus and zona incerta projection populations. Established a high-throughput method to transcriptionally define projection neurons, VECTORseq, that reimagines transgenes expressed by widely used retrogradely infecting viruses as multiplexed RNA barcodes that are detected in single-cell sequencing.
2021-12-02 | GSE189907 | GEO
Project description:Identification of Viruses Infecting Lily in Korea by Nanopore sequencing
Project description:Implementation and evaluation of tests (PCR, ELISA, NGS) for the presence of viruses, viroids and phytoplasma in propagation materials of apple. Research and application of new sanitation techniques using promising antiviral substances, or cryo-knife. Confirmation of health status of sanitated cultivars including use of modern diagnostic methods such as high-throughput sequencing (NGS).
2022-12-09 | GSE205183 | GEO
Project description:Identification of viruses in five major rivers in Korea by high-throughput sequencing
| PRJNA1112127 | ENA
Project description:Identification of viruses infecting Citrus in Korea by nanopore sequencing technology
| PRJNA957239 | ENA
Project description:Identification of viruses infecting tulip in Korea by ONT nanopore sequencing
| PRJNA1127789 | ENA
Project description:Identification of viruses infecting Narcissus pseudonarcissus in Korea by ONT nanopore sequencing
