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TXNIP loss expands Myc transcriptome

ABSTRACT: TXNIP loss expands Myc transcriptome

PROVIDER: PRJNA923027 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23054920_1.fastq.gz	Fastqsanger.gz
	SRR23054920_2.fastq.gz	Fastqsanger.gz
	SRR23054921_1.fastq.gz	Fastqsanger.gz
	SRR23054921_2.fastq.gz	Fastqsanger.gz
	SRR23054922_1.fastq.gz	Fastqsanger.gz

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TXNIP loss expands Myc transcriptome [RNA-seq]

Project description:TXNIP loss expands Myc transcriptome [RNA-seq]

| PRJNA859857 | ENA

TXNIP loss expands Myc transcriptome

Project description:To examine the generality of our finding where the gene expression profile of TXNIP knockout in MDA-MB-231 cells resembles of that Myc overexpression transcriptional program, our lab has generated TXNIP null HCC70 (HCC70:TKO) and MB135 (MB135:TKO) cells. We characterized the gene exrpession programs of these cells by RNA-seq.

2023-02-07 | GSE222693 | GEO

TXNIP loss expands Myc transcriptome [RNA-seq]

Project description:To better understand how TXNIP in the contributes to the poor prognosis of triple-negative breast cancers, our lab has generated TXNIP null MDA-MB-231 cells (231:TKO). We characterized the gene exrpession programs of these cells by RNA-seq. To determine the Myc-dependence of the transcriptional programs of 231:TKO cells, we used an siRNA RNA approach to reduce Myc levels.

2023-02-07 | GSE208415 | GEO

TXNIP loss increases Myc genomic occupancy [ChIP-seq]

Project description:Our RNA sequencing result demonstrated that TXNIP loss increased the levels of Myc-dependent transcription. To determine whether TXNIP regulates global Myc genomic occupancy, we performed Myc ChIP-seq on parental 231 and 231:TKO cells.

2023-02-07 | GSE208412 | GEO

TXNIP loss does not increase chromatin accessibility for Myc binding.

Project description:Our Myc ChIP sequencing result demonstrated that TXNIP loss increased Myc genomic occupancy levels. To determine whether TXNIP loss increased Myc genomic occupancy through increasing chromatin accessibility for Myc binding, we performed assay for transposase-accessible chromatin with sequencing (ATAC-seq) on parental 231 and 231:TKO cells.

2023-02-07 | GSE222672 | GEO

TXNIP loss

Project description:TXNIP loss

| PRJNA923024 | ENA

TXNIP loss increases Myc genomic occupancy [ChIP-seq]

Project description:TXNIP loss increases Myc genomic occupancy [ChIP-seq]

| PRJNA859844 | ENA

TXNIP loss does not increase chromatin accessibility for Myc binding.

Project description:TXNIP loss does not increase chromatin accessibility for Myc binding.

| PRJNA922999 | ENA

TXNIP loss expands Myc-dependent transcriptional programs by increasing Myc genomic binding.

Project description:The c-Myc protooncogene places a demand on glucose uptake to drive glucose-dependent biosynthetic pathways. To meet this demand, c-Myc protein (Myc henceforth) drives the expression of glucose transporters, glycolytic enzymes, and represses the expression of thioredoxin interacting protein (TXNIP), which is a potent negative regulator of glucose uptake. A Mychigh/TXNIPlow gene signature is clinically significant as it correlates with poor clinical prognosis in triple-negative breast cancer (TNBC) but not in other subtypes of breast cancer, suggesting a functional relationship between Myc and TXNIP. To better understand how TXNIP contributes to the aggressive behavior of TNBC, we generated TXNIP null MDA-MB-231 (231:TKO) cells for our study. We show that TXNIP loss drives a transcriptional program that resembles those driven by Myc and increases global Myc genome occupancy. TXNIP loss allows Myc to invade the promoters and enhancers of target genes that are potentially relevant to cell transformation. Together, these findings suggest that TXNIP is a broad repressor of Myc genomic binding. The increase in Myc genomic binding in the 231:TKO cells expands the Myc-dependent transcriptome we identified in parental MDA-MB-231 cells. This expansion of Myc-dependent transcription following TXNIP loss occurs without an apparent increase in Myc's intrinsic capacity to activate transcription and without increasing Myc levels. Together, our findings suggest that TXNIP loss mimics Myc overexpression, connecting Myc genomic binding and transcriptional programs to the nutrient and progrowth signals that control TXNIP expression.

| S-EPMC10058090 | biostudies-literature

Secretome from TXNIP knockout human colorectal cell line (DLD1)

Project description:Chemotherapy, the standard of care treatment for cancer patients with advanced disease, has been increasingly recognised to activate host immune responses to produce durable outcomes. Here, in colorectal adenocarcinoma (CRC) we identify oxaliplatin-induced Thioredoxin Interacting Protein (TXNIP), a MondoA-dependent tumor suppressor gene, as a negative regulator of Growth/Differentiation Factor 15 (GDF15). GDF15 is a negative prognostic factor in CRC and promotes the differentiation of regulatory T cells (Tregs), which inhibit CD8 T cell activation. Intriguingly, multiple models including patient-derived tumor organoids demonstrate that the loss of TXNIP and GDF15 responsiveness to oxaliplatin is associated with advanced disease or chemotherapeutic resistance, with transcriptomic or proteomic GDF15/TXNIP ratios showing potential as a prognostic biomarker. These findings illustrate a potentially common pathway where chemotherapy-induced epithelial oxidative stress drives local immune remodelling for patient benefit, with disruption of this pathway seen in refractory or advanced cases.

2024-08-12 | PXD051666 | Pride

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