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H3K4me2/3 modulate the stability of RNA polymerase II pausing (PRO-Seq)

ABSTRACT: H3K4me2/3 modulate the stability of RNA polymerase II pausing (PRO-Seq)

PROVIDER: PRJNA923652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23074025_1.fastq.gz	Fastqsanger.gz
	SRR23074025_2.fastq.gz	Fastqsanger.gz
	SRR23074026_1.fastq.gz	Fastqsanger.gz
	SRR23074026_2.fastq.gz	Fastqsanger.gz

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H3K4me2/3 modulate the stability of RNA polymerase II pausing

Project description:H3K4me2/3 modulate the stability of RNA polymerase II pausing

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H3K4me2/3 modulate the stability of RNA polymerase II pausing (PRO-Seq)

Project description:Modifications of histones are intricately linked with the regulation of gene expression, with demonstrated roles in various physiological processes and disease pathogenesis. Methylation of histone H3 lysine 4 (H3K4), implemented by the COMPASS family, is enriched at promoters and associated cis-regulatory elements, with H3K4 trimethylation (H3K4me3) considered a hallmark of active gene promoters. However, the relative roles of deposition and removal of H3K4 methylation, as well as the extent to which these events contribute to transcriptional regulation have so far remained unclear. Here, through rapid depletion of either of two shared subunits of COMPASS family members, we reveal a dynamic turnover of H3K4me2 and H3K4me3 mediated by the KDM5 family of histone demethylases. Loss of H3K4me2 and H3K4me3 following COMPASS disruption does not impair the recruitment of TFIID and initiating RNA polymerase II (Pol II). Instead, their loss leads to reductions in the paused form of Pol II on chromatin while inducing the relative enrichment of the Integrator-PP2A (INTAC) termination complex, leading to reduced levels of elongating polymerases, thus revealing how H3K4me2 and H3K4me3 dynamics can regulate Pol II pausing to sustain or attenuate transcription.

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H3K4me2/3 modulate the stability of RNA polymerase II pausing (ChIP-Seq)

Project description:H3K4me2/3 modulate the stability of RNA polymerase II pausing (ChIP-Seq)

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H3K4me2/3 modulate the stability of RNA polymerase II pausing (RNA-Seq)

Project description:H3K4me2/3 modulate the stability of RNA polymerase II pausing (RNA-Seq)

| PRJNA923651 | ENA

H3K4me2/3 modulate the stability of RNA polymerase II pausing (RNA-Seq)

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H3K4me2/3 modulate the stability of RNA polymerase II pausing.

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Dissecting polymerase pausing with TV-PRO-seq

Project description:Dissecting polymerase pausing with TV-PRO-seq

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Dissecting polymerase pausing with TV-PRO-seq

Project description:Transcription of many genes in metazoans is subject to polymerase pausing, which corresponds to the transient arrest of transcriptionally engaged polymerase. It occurs mainly at promoter proximal regions and is not well understood. In particular, a genome-wide measurement of pausing times at high resolution has been lacking. This is because relevant experimental techniques are mostly based on average polymerase occupancies across many cells which cannot discriminate between few slow polymerases and many quick polymerases at a position, since the data corresponds to the average of many cells. We present here an extension of the PRO-seq assay, which we termed time variant PRO-seq (TV-PRO-seq)based on a detailed analysis of the logics underlying PRO-seq, that achieves this goal.

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H3K4me2/3 modulate the stability of RNA polymerase II pausing

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