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Differential transcriptome analysis of naïve T cell activtion, TH1 cell differentiation and TH1 cell reactivation

ABSTRACT: Differential transcriptome analysis of naïve T cell activtion, TH1 cell differentiation and TH1 cell reactivation

PROVIDER: PRJNA923806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23080757_1.fastq.gz	Fastqsanger.gz
	SRR23080757_2.fastq.gz	Fastqsanger.gz
	SRR23080758_1.fastq.gz	Fastqsanger.gz
	SRR23080758_2.fastq.gz	Fastqsanger.gz
	SRR23080759_1.fastq.gz	Fastqsanger.gz

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Differential transcriptome analysis of naïve T cell activtion, TH1 cell differentiation and TH1 cell reactivation

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Project description:ATAC-Seq experiments were performed to elucidate the chromatin state changes among naïve CD4+ T cells, WT follicular helper T (TFH) cells and WT type 1 helper T (TH1) cells Day2 (D2), Day5 (D5), Day8 (D8) post-LCMV-Armstrong infection, as well as EZH2-null (KO) TFH and TH1 at Day8 post-LCMV-infection. The analysis suggested stringent lineage-specific mode of chromatin accessibility in each group, indicating chromatin remodeling is tightly associated with TFH versus TH1 lineage differentiation in response to acute viral infection. Furthermore, the comparison between wild-type and EZH2-null TFH cells showed that less-opening state of certain chromatin accessible region in TFH-differentiation associated genes in the formers, suggesting EZH2 led to permissive chromatin accessibility primarily at specific regions of TFH-associated genes. H3K27me3-ChIP-seq was performed in WT TFH and TH1 cells to confirm the deposition of the histone marks at those loci.

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