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RNA-seq of in vitro differentiated naïve and Th9 cells

ABSTRACT: RNA-seq of in vitro differentiated naïve and Th9 cells

PROVIDER: PRJNA923819 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23082626_1.fastq.gz	Fastqsanger.gz
	SRR23082626_2.fastq.gz	Fastqsanger.gz
	SRR23082627_1.fastq.gz	Fastqsanger.gz
	SRR23082627_2.fastq.gz	Fastqsanger.gz
	SRR23082628_1.fastq.gz	Fastqsanger.gz

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RNA-seq of in vitro differentiated naïve and Th9 cells

Project description:To investigate changes in gene expression over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed gene expression profiling analysis using data obtained from RNA-seq in cells from 2 different human donors and from 2 mice at 4 time points.

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ChIP-seq of in vitro differentiated naïve and Th9 cells

Project description:To investigate changes in chromatin accessibility over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K4me1, K3K4me3, and H3K27Ac in cells from 2 different human donors and from 2 mice at 4 time points.

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Tumor rejection in Cblb-/- mice depends on IL-9 and Th9 cells

Project description:Casitas B lymphoma-b (Cbl-b) is a central negative regulator of cytotoxic T and NK cells, explaining its intracellular checkpoint function in cancer. Increasing evidence highlights the importance of CD4+ T helper cell populations (e.g., IL-9 producing T cells: Th9 cells) for anti-cancer immunity. We here provide experimental evidence that Cbl-b acts as rheostat favoring regulatory T cells (Treg) at the expense of Th9 cell differentiation. Adoptively transferred tumor-model antigen specific Cblb-/- Th9 cells exert superior anti-tumor activity leading to improved melanoma control in vivo. Accordingly, blocking IL-9 in melanoma cell-exposed Cblb-/- mice reverses their tumor rejection phenotype. Single cell RNA sequencing of in vitro differentiated Th9 cells from naïve T cells isolated from WT and Cblb-/- animals revealed the transcriptomic basis for increased Th9 cells differentiation. In summary, we here first establish IL-9 and Th9 cells as key anti-tumor executers in Cblb-/- animals. This knowledge may be helpful for future improvement of adoptive T cell therapies in cancer.

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