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Histone bivalency regulates the timing of cerebellar granule cell development in vivo [ChIP-seq]

ABSTRACT: Histone bivalency regulates the timing of cerebellar granule cell development in vivo [ChIP-seq]

PROVIDER: PRJNA926530 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23193200.fastq.gz	Fastqsanger.gz
	SRR23193201.fastq.gz	Fastqsanger.gz
	SRR23193202.fastq.gz	Fastqsanger.gz
	SRR23193203.fastq.gz	Fastqsanger.gz
	SRR23193204.fastq.gz	Fastqsanger.gz

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Histone bivalency regulates the timing of cerebellar granule cell development in vivo [RNA-seq]

Project description:Developing neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature, multipolar neurons. Here we use RNA-seq and ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron, the mouse cerebellar granule cell (GC). We find that in proliferating GC progenitors, H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Inhibiting H3K27 methyltransferases EZH1 and EZH2 in vitro and in organotypic cerebellar slices dramatically altered the expression of bivalent genes and induced the downregulation of migration-related genes and upregulation of synaptic genes, inhibited glial-guided migration and accelerated dendrite formation. These data show that histone bivalency is required to regulate the timing of the progression from progenitor cells to mature neurons.

2023-02-06 | GSE223486 | GEO

Histone bivalency regulates the timing of cerebellar granule cell development in vivo [ChIP-seq]

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Histone bivalency regulates the timing of cerebellar granule cell development in vivo.

Project description:Histone bivalency regulates the timing of cerebellar granule cell development in vivo.

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Histone bivalency regulates the timing of cerebellar granule cell development in vivo [RNA-seq]

Project description:Histone bivalency regulates the timing of cerebellar granule cell development in vivo [RNA-seq]

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Histone bivalency regulates the timing of cerebellar granule cell development.

Project description:Developing neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature neurons. Here we used RNA-seq and H3K4me3 and H3K27me3 ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron: the mouse cerebellar granule cell (GC). We found that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Expressing a fluorescent sensor for bivalent domains revealed subnuclear bivalent foci in proliferating GCPs. Inhibiting H3K27 methyltransferases EZH1 and EZH2 in vitro and in organotypic cerebellar slices dramatically altered the expression of bivalent genes, induced the down-regulation of migration-related genes and up-regulation of synaptic genes, inhibited glial-guided migration, and accelerated terminal differentiation. Thus, histone bivalency is required to regulate the timing of the progression from progenitor cells to mature neurons.

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Histone bivalency regulates the timing of cerebellar granule cell development in vivo.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Gene expression of cerebellar granule cell layer

Project description:We performed gene-expression analysis of mouse cerebellar granule cell layer as compared to that of Purkinje cells. DNA microarray analysis detected genes in cerebellar granule cell layer, most of which are classified into functional molecule categories. Our comparative analysis between Purkinje cells and the granule cell layer showed that the characteristic expression pattern in Purkinje cells was particularly represented by M-bM-^@M-^\the neural communication systemM-bM-^@M-^] components. Pukinje cells and granule cell layer of the mouse cerebellum were collected by laser microdissection for RNA extraction and hybridization on Affymetrix microarrays.

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Project description:To establish a new model system with which to study cerebellar granule lienage development and disease, human hbNES cells were differentiated to cerebellar granule neurons over a 56 day period.

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