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Effect of TIM-3 knockout in the tumoroginesis in the NP53 DMG cell line

ABSTRACT: Effect of TIM-3 knockout in the tumoroginesis in the NP53 DMG cell line

PROVIDER: PRJNA928687 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23253173_1.fastq.gz	Fastqsanger.gz
	SRR23253173_2.fastq.gz	Fastqsanger.gz
	SRR23253174_1.fastq.gz	Fastqsanger.gz
	SRR23253174_2.fastq.gz	Fastqsanger.gz
	SRR23253175_1.fastq.gz	Fastqsanger.gz

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Effect of TIM-3 knockout in the tumoroginesis in the NP53 DMG cell line

Project description:To investigate the function of TIM-3 expression in the regulation of NP53 proliferation and tumorigenesis differentiation, we established DMG cell lines in which each target gene has been knocked out by CRISPR/Cas9.

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Microarray analysis of the effect of shRNA-mediated HAVCR2 knock-down (KD) in TIM-3-positive AML cell line, KASUMI-3

Project description:Purpose: To clarify the function of TIM-3 signaling in AML cells. Analysis of lentiviral shRNA-mediated knockdown of TIM-3 in TIM-3+ acute myeloid leukemia (AML) cell line, KASUMI-3. Consistent with our previous study (Kikushige et al., Cell Stem Cell 2015) revealing the presence of TIM-3/galectin-9 autocrine loop and its constitutive signaling in AML, KD of receptor,TIM-3, alone significantly impaired the proliferation of KASUMI-3 in vitro and in vivo.

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Effect of FAM50 knockout on the transcriptome

Project description:A knockout clone has been generated for both FAM50A and FAM50B; knockout of the other gene is then performed and the transcriptome is analysed to look at the effect of dual gene loss.

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Project description:The effect of TIM-3 stimulation was studied on B16F10 mouse melanoma cells, in vitro.

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Project description:Effect of Rgnef knockout in an aggressive ovarian cancer cell line

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microRNA expression in Tim-3 knockdown RAW264.7 cell

Project description:RAW264.7 cell was stably transfected with Tim-3 siRNA(SI) or negative control(NC) and were analyzed for microRNA expression Differerently expressed microRNA in Tim-3 knockdown RAW264.7 cells were summarized and were used for further analysis RAW264.7 cells stably transfected with Tim-3 siRNA(SI) or negative control(NC) and were used to collect microRNA without any other treatment

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Histone H3 wild-type DIPG/DMG overexpressing EZHIP extend the spectrum diffuse midline gliomas with PRC2 inhibition beyond H3-K27M mutation

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