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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

ABSTRACT: The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

PROVIDER: PRJNA929269 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23266832.fastq.gz	Fastqsanger.gz
	SRR23266833.fastq.gz	Fastqsanger.gz
	SRR23266834.fastq.gz	Fastqsanger.gz
	SRR23266835.fastq.gz	Fastqsanger.gz
	SRR23266836.fastq.gz	Fastqsanger.gz

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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not completely understood. Here, we have studied antigen-specific assay systems, which revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors such as Amphiregulin (Areg). Tissue remodeling could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN- and TNF. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with an effector program promoting Areg, TNF and IFNg expression, and a TCR-linkage analysis demonstrated a strong clonal relation between tissue and blood PD1+TIGIT+TOX+ CD8 T cells with tissue remodeling abilities. This regenerative program was also present in anti-cancer associated CD8 populations including chimeric antigen receptor (CAR) CD8 T cells. Once activated, the tissue regenerative program of human CD8 T cells enhanced tumor spheroid, as well as primary liver/bile duct organoid growth. Our findings may help to understand the conflicting CD8 biology in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies for solid tumors.

2023-11-20 | GSE223987 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

Project description:The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

| PRJNA929268 | ENA

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

2023-11-20 | GSE223988 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not understood. Here, antigen-specific assay systems revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors. Tissue remodeling by CD8 T cells could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN-Ã¯ÂÂ§ and TNF. The regenerative program also enhanced tumor spheroid and primary organoid growth. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with a strong TCR clonal relation to blood PD1+TIGIT+ CD8 T cells with tissue remodeling abilities. Our findings may help to understand CD8 in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies.

| EGAS00001006960 | EGA

Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells.

Project description:Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells. Keywords: cell type comparison, IFN gamma treatment.

2008-07-01 | GSE10880 | GEO

Transcription profiling of human MRC5, IFN gamma treated MRC5 and PGF cells.

Project description:Expression profiling of MRC5, IFN gamma treated MRC5 and PGF cells. Experiment Overall Design: MRC5 cells were treated for 24 hours with IFN gamma (200 IU/ml) and their expression profile was compared to untreated MRC5 and PGF cells.

2008-10-20 | E-GEOD-10880 | biostudies-arrayexpress

Transcriptome of MRC5 fibroblasts

Project description:Purpose: to investigate the human transcriptome in MRC5 fibroblasts

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Genomic alteration in MRC5-derived induced pluripotent stem (MRC5-iPS) cell line, Tic

Project description:Disease-specific induced pluripotent stem (iPS) cells have been used for a model to analyze pathogenesis of the disease. We generated iPS cells derived from a fibroblastic cell line of ataxia telangiectasia (AT-iPS cells). In analysis of AT-iPS cells, the human wild-type iPS cell line (MRC5-iPS) was generated and cultured in the same conditions as the diseased iPS cell lines. It is an ideal control cell line for the disease and patient-specific iPS cell lines. Because MRC5-iPS cells exhibited considerable chromosomal abnormalities in vitro, we performed a structural alteration analysis by using a SNP genotyping array for MRC5-iPS cell line, Tic, at passage 15, passage 30, and passage 37.

2013-07-23 | GSE49082 | GEO

Transcriptome of MRC5 fibroblasts

Project description:Transcriptome of MRC5 fibroblasts

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RNAi - MBD2 - MRC5 cells

Project description:Relationship between MBD2 depletion and gene expression in a normal human fibroblastic cell line, MRC5

2011-09-16 | E-MEXP-2916 | biostudies-arrayexpress