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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

ABSTRACT: The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

PROVIDER: PRJNA929268 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23266850.fastq.gz	Fastqsanger.gz
	SRR23266851.fastq.gz	Fastqsanger.gz
	SRR23266852.fastq.gz	Fastqsanger.gz
	SRR23266853.fastq.gz	Fastqsanger.gz
	SRR23266854.fastq.gz	Fastqsanger.gz

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The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (HaCaT)

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not completely understood. Here, we have studied antigen-specific assay systems, which revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors such as Amphiregulin (Areg). Tissue remodeling could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN- and TNF. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with an effector program promoting Areg, TNF and IFNg expression, and a TCR-linkage analysis demonstrated a strong clonal relation between tissue and blood PD1+TIGIT+TOX+ CD8 T cells with tissue remodeling abilities. This regenerative program was also present in anti-cancer associated CD8 populations including chimeric antigen receptor (CAR) CD8 T cells. Once activated, the tissue regenerative program of human CD8 T cells enhanced tumor spheroid, as well as primary liver/bile duct organoid growth. Our findings may help to understand the conflicting CD8 biology in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies for solid tumors.

2023-11-20 | GSE223988 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

Project description:The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

| PRJNA929269 | ENA

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling (MRC5)

2023-11-20 | GSE223987 | GEO

The effector program of human CD8 T cells can promote both target cell killing and tissue remodeling

Project description:CD8 T lymphocytes are classically viewed as cytotoxic T cells. Whether human CD8 T cells can, in parallel, induce a regenerative program is not understood. Here, antigen-specific assay systems revealed that human CD8 T cells not only mediated cytotoxicity, but also promoted bystander fibroblast and epithelial cell activation, leading to the production of tissue regenerative factors. Tissue remodeling by CD8 T cells could be inhibited by blocking the epidermal growth factor receptor or the effector cytokines IFN-Ã¯ÂÂ§ and TNF. The regenerative program also enhanced tumor spheroid and primary organoid growth. Using single-cell chromatin accessibility and gene expression analysis, we identified a tissue-resident CD8 T cell population in healthy donor skin and adipose tissue with a strong TCR clonal relation to blood PD1+TIGIT+ CD8 T cells with tissue remodeling abilities. Our findings may help to understand CD8 in the tumor micromilieu and become relevant for the design of CD8 T-cell therapies.

| EGAS00001006960 | EGA

The effects of SNAI2 overexpression on the transcriptional program in HaCaT cells

Project description:Investigation of the transcriptional profile of SNAI2-overexpressing HaCaT cells.

2021-09-01 | GSE166199 | GEO

The effects of TGF-β1-induced EMT on the transcriptional program in HaCaT cells

Project description:Investigation of the transcriptional profile of TGF-β1-induced EMT in HaCaT cells.

2021-09-01 | GSE166200 | GEO

Protein dataset of immortalized HaCaT cells p53 knock down HaCaT keratinocytes

Project description:The p53 protein is encoded by TP53 gene and plays the key role in significant number of cellular processes including proliferation, apoptosis and regulation of many stress response pathways. P53 acts like a direct transcription activator of numerous genes regulating cell cycle arrest, DNA repair, growth inhibition and many others (Mollereau and Ma, 2014). The canonical biological function of p53 is maintaining genome integrity via elimination of damaged or exposed to genotoxic stress cells. Immortalized HaCaT cells are widely used for keratinocyte research, since they maintain stable keratinocyte phenotype, have nearly unlimited proliferative potential, do not require specific growth and differentiation factors (Colombo et al., 2017). Also, HaCaT cells produce typical differentiation markers such as cytokeratins K14 and K10, involucrin (Colombo et al., 2017) and respond to keratinocyte differentiation stimuli. Taking together, HaCaT cells have similar to normal human keratinocytes (NHK) properties, however, as many of spontaneously immortalized cell lines HaCaT cells bear two mutant p53 alleles - R282Q and H179Y (Lehman et al., 1993). Mutp53 in HaCaT has an increased affinity to other p53 family members (p63, p73), which significantly expands p53 properties. Moreover, mutp53 indirectly affects specific target genes via protein-protein interactions with other transcription factors (NF-Y, E2F1, NF-KB) or by tethering p63 to new promotor locations. For more detailed investigation of mutp53 impact on various processes in HaCaT cells we performed a shRNA mediated knockdown of mutp53. For generation of stable TP53 knockdown we employed plasmid vector pLKO-p53-shRNA-941 (Addgene # #25637) followed by puromycin selection of transduced cells. Here we present proteomic dataset obtained from wild type HaCaT cells and p53 knock down HaCaT keratinocytes.

2022-04-28 | PXD033538 | Pride

Next Generation Sequencing Facilitates Quantitative Analysis of HaCaT and OSM-treated HaCaT Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between HaCaT cell and OSM-treated HaCaT cells Methods: mRNA profiles of HaCaT cells and OSM-treated HaCaT cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.

2021-03-18 | GSE150994 | GEO

The N6-methyadenosine (m6A) levels in HaCaT-T cells and control HaCaT cells

Project description:Human m6A-mRNA&lncRNA Epitranscriptomic Microarray of arsenite-transformed human keratinocytes (HaCaT-T cells, 1 μM arsenite exposure for 50 passages) compared to its control HaCaT cells (passed for 50 passages without arsenic exposure).

2020-11-08 | GSE161033 | GEO

RNA-seq of Hacat cells treated with TOPK inhibitor

Project description:Hacat cells were treated with TOPK inhibitor to elucidate the effect of TOPK signaling on Hacat cells

2024-01-18 | GSE253203 | GEO