Project description:The objective is to analyze the differential expression between the wild strain and a pSCL4- S. clavuligerus mutant Experiment type Expression profiling by array
Project description:The RNA sequencing approach was utilized to carry out a comparative transcriptome analysis between Streptomyces hygroscopicus 5008 wild-type and a genetically engineered strain. The A-factor-like cascade play an important role in the regulation of validamycin biosynthesis by Streptomyces hygroscopicus 5008, and the pleiotropic regulator AdpA-H may positively regulate the transcription of gene cluster for the biosynthesis. shbR1 and shbR3 as the A-factor receptor homolog genes, could repress the transcription of AdpA-H. By tandem deletions of these genes, the production and productivity of validamcyin was significantly enhanced. To explore the effects of the shbR1/R3 double deletion of the overall cellular metabolism, the RNA sequencing approach was utilized to carry out a comparative transcriptome analysis between wild-type and shbR1/shbR3 double mutant (genetically engineered strain).
Project description:To discover novel regulators that influence avermectin biosynthesis, comparative transcriptome analysis between wild-type strain ATCC31267 and avermectin overproducing strain 76-02-e were performed to reveal some differentially expressed genes.
Project description:The terminal compartments of Streptomyces are less prone to transcription than the rest of the chromosome. Indeed, the expression of the highly variable regions enriched in those compartments is generally conditional and often requires an empirical approach to characterize the inducing conditions. For instance, in the context of identifying adequate antibiotic production conditions, an OSMAC (“One Strain Many Compounds”) approach is frequently implemented, based on strain cultivation in different environmental conditions (composition of the medium, growth time, temperature, co-cultures, etc.). Likewise, to find the expression conditions of a complete prophage of Streptomyces ambofaciens ATCC 23877 (named 'Samy' phage/prophage), we conducted a similar approach by analyzing the transcriptomes in five solid media (HT, SAF, ONA, MMM, MMM+NAG). The terminal compartments of Streptomyces are less prone to transcription than the rest of the chromosome. Indeed, the expression of the highly variable regions enriched in those compartments is generally conditional and often requires an empirical approach to characterize the inducing conditions. For instance, in the context of identifying adequate antibiotic production conditions, an OSMAC (“One Strain Many Compounds”) approach is frequently implemented, based on strain cultivation in different environmental conditions (composition of the medium, growth time, temperature, co-cultures, etc.). Likewise, to find the expression conditions of a complete prophage of Streptomyces ambofaciens ATCC 23877 (named 'Samy' phage/prophage), we conducted a similar approach by analyzing the transcriptomes in five solid media (HT, SAF, ONA, MMM, MMM+NAG).
Project description:Change in gene expression for a wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strain (UCD 543) of Nostoc punctiforme ATCC 29133 over the time course of hormogonium development This study is further descirbed in Risser, D.D. and Meeks, J.C. 2013. Comparative transcriptomics with a motility deficient mutant leads to identification of a novel polysaccharide secretion system in Nostoc punctiforme. Molecular Microbiology
Project description:To increase production of the important pharmaceutical compounds, both mutagenesis approaches and rational engineering have been extensively applied. Mutagenesis approaches are most popular in industry, but their effects have not yet been studied very well. Here, we used microarrays to compare the transcriptomes of the S. clavuligerus wild type (ATCC 27064) strain and the DS48802 clavulanic acid high-producer strain, which has been obtained by classical strain improvement (mutagenesis).
Project description:The objective was to analyze the differential expression between the wild strain and the Streptomyces clavuligerus ΔclaR::aac mutant
