Project description:Comparison of transcript abundance between control (untreated) and methotrexate treated S3 Drosophila cells and ovaries disected from female flies. Keywords: Stress response
Project description:Comparison of transcript abundance between control (untreated) and methotrexate treated S3 Drosophila cells and ovaries disected from female flies. S3 cells were exposed to 0 or 5.2 X 10^-8 M MTX for 4 days then harvested. Flies were exposed to 0 or 5 ppm MTX for 5 days then ovaries were dissected. RNA was extracted from S3 cells and ovaries. 4 S3 microarrays with one dye-swap and 2 ovary microarrays swapping dyes were hybridized to spotted cDNA microarrays.
Project description:H3K27me3 profiles using Cleavage under targets and Release using nuclease (Cut&Run) in control and KD Drosophila melanogaster ovaries. We examined the impact on chromatin profiles in Drosophila melanogaster ovaries in which the lid, the Sin3a, the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissue-specific shRNA expression. We additionally explored H3K27me3 and H3K9me3 in control and dhd mutant ovaries either carrying or not a transgene.
Project description:We performed mRNA transcriptional profiling on Drosophila S3 cells after 4 hours treatment with novel lipid storage inhibitors belonging to three different chemotypes.
