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Triptolide analogs induced apoptosis on pancreatic cancer patient-derived organoids

ABSTRACT: Triptolide analogs induced apoptosis on pancreatic cancer patient-derived organoids

PROVIDER: PRJNA933573 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23396707_1.fastq.gz	Fastqsanger.gz
	SRR23396707_2.fastq.gz	Fastqsanger.gz
	SRR23396708_1.fastq.gz	Fastqsanger.gz
	SRR23396708_2.fastq.gz	Fastqsanger.gz
	SRR23396709_1.fastq.gz	Fastqsanger.gz

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Triptolide analogs induced apoptosis on pancreatic cancer patient-derived organoids

Project description:Investigate the MOA of triptolide analog-CK21 in pancreatic cancer. Time course transcriptomic profiling of tumor organoids treated with CK21 in vitro revealed <10 differentially expressed genes (DEGs) at 3 h and ~8,000 DEGs at 12 h. Overall inhibition of general RNA transcription was observed, and Ingenuity pathway analysis together with functional cellular assays confirmed inhibition of the NF-κB pathway, increased oxidative phosphorylation and mitochondrial dysfunction, leading ultimately to increased reactive oxygen species (ROS) production, reduced B-cell-lymphoma protein 2 (BCL2) expression, and mitochondrial-mediated tumor cell apoptosis.

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Triptolide sensitizes cancer cells to nucleoside DNA methyltransferase inhibitors through inhibition of DCTPP1 mediated cell-intrinsic resistance [Array]

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Transcriptome of cancer associated fibroblasts before and after triptolide treatment in pancreatic cancer

Project description:Pancreatic cancer is characterized by heavy desmoplasia. Triptolide and its water-soluble pro-drug Minnelide are extremely efficient against pancreatic cancer in animal models. However, the effects of triptolide on pancreatic cancer stromal cells are largely unknown. The aim of this project is to indentify potential cellular functions that are affected by triptolide in pancreatic cancer associated fibroblasts.

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Project description:Triptolide or triptonide, major bioactive components isolated from the traditional Chinese herb Tripterygium wilfordii Hook f. (TWHF), have been shown to exert various pharmacological effects on systemic autoimmune disease. However, the severe toxicity, especially reproduction toxicity, of triptolide or triptonide prevents wide clinical use for those people with fertility needs. Long noncoding (lnc) RNAs are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide or triptonide -induced reproduction toxicity is unknown. Here we found that exposure of spermatocyte cell line (GC2) to triptolide or triptonide led to reduction in cell viability and induced cell apoptosis and autophagy. Then the total RNAs of triptolide or triptonide -treated GC2 cells were used to investigate lncRNA expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide or triptonide-induced toxicity. Gene ontology and pathway analyses showed that the functions of differentially expressed lncRNA targets and mRNAs were closely linked with many processes involved in spermatogenesis.

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Glyco-gene expression in human breast cancer MDA-MB-231 cells treated with ManNAc analogs

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Triptolide Targets Super-Enhancer Networks in Pancreatic Cancer Cells and Cancer Associated Fibroblasts

Project description:This study invesitgated the super-enhancer (SE) networks in pancreatic cancer cells and cancer associated fibroblasts (CAFs) as well as the effects of triptolide treatment on the SEs and the expression of the SE associated genes using ChIP-Seq and RNA-Seq.

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The genes affected by Triptolide in activated mouse primary T cells

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