Project description:The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia.
Project description:Here, we report analysis of both the bacterial and host transcriptome as affected by colonization of R. hominis in the mouse gut. Microbial genes required for colonization and adaptation in the murine gut, as well as host genes responding to colonization by this bacterial species, were uncovered.
Project description:GWAT store most of the TAG in mice, ob/ob mice is an obese mice. Ob/ob/Fsp27-/- mice are lean when compared with ob/ob mice. The GWAT weight was dramatically reduced in ob/ob/Fsp27-/- mice. We next extract the total RNA from the GWAT of ob/ob and ob/ob/Fsp27-/- mice, to perform microaary analysis using Mouse Genome 430 2.0 arrays, Affymetrix. We then analysised the up-regulated and down regulated pathways.
Project description:GWAT store most of the TAG in mice, ob/ob mice is an obese mice. Ob/ob/Fsp27-/- mice are lean when compared with ob/ob mice. The GWAT weight was dramatically reduced in ob/ob/Fsp27-/- mice. We next extract the total RNA from the GWAT of ob/ob and ob/ob/Fsp27-/- mice, to perform microaary analysis using Mouse Genome 430 2.0 arrays, Affymetrix. We then analysised the up-regulated and down regulated pathways. We extract the RNA of GWAT from 4 month old mice and hybridization on Affymetrix microarrays. We then analysis the data.