Dataset Information

Shotgun proteomics of Roseburia grown on human milk oligosaccharides and of Roseburia grown on mucin in co-culture with Akkermansia muciniphilia.

ABSTRACT: The main objective of the present proteomic study is to identify the metabolic response, in particular theglycan uptake and degradation machinery, conferring members of Roseburia growth on HMOs and/or onrelated O-glycans. Accordingly, the proteomes of R. hominis and R. inulinivorans bothgrown on humanmilk oligosaccharides (HMOs), were compared to glucose to reveal the molecular basis for growth onHMOs. Furthermore, we compare the proteomes of R. hominis and R. inulinivorans grownin co-culturewith A. muciniphilia either on mucin or on glucose to identify potential metabolic routes of mucin derivedO-glycan utilization in Roseburia.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Akkermansia Muciniphila Atcc Baa-835 Roseburia Hominis A2-183 Roseburia Inulinivorans Dsm 16841

TISSUE(S): Gut

SUBMITTER: Michael Pichler

LAB HEAD: Maher Abou Hachem

PROVIDER: PXD015045 | Pride | 2020-07-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180815_FJ_LHB_Mitchi_Glucose1.raw	Raw
	20180815_FJ_LHB_Mitchi_Glucose2.raw	Raw
	20180815_FJ_LHB_Mitchi_Glucose3.raw	Raw
	20180815_FJ_LHB_Mitchi_Glucose4.raw	Raw
	20180815_FJ_LHB_Mitchi_LNT1.raw	Raw

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Publications

Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways.

Pichler Michael Jakob MJ Yamada Chihaya C Shuoker Bashar B Alvarez-Silva Camila C Gotoh Aina A Leth Maria Louise ML Schoof Erwin E Katoh Toshihiko T Sakanaka Mikiyasu M Katayama Takane T Jin Chunsheng C Karlsson Niclas G NG Arumugam Manimozhiyan M Fushinobu Shinya S Abou Hachem Maher M

Nature communications 20200703 1

The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support the growth of Roseburia and Eubacterium members on distinct human milk oligosaccharides (HMOs). The HMO pathways, which ...[more]

PMID: 32620774

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