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Transcriptional profiling of THP-1 monocytes conditioned with Lacticaseibacilli rhamnosus R0011 secretome

ABSTRACT: Transcriptional profiling of THP-1 monocytes conditioned with Lacticaseibacilli rhamnosus R0011 secretome

PROVIDER: PRJNA934875 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of THP-1 monocytes conditioned with Lacticaseibacilli rhamnosus R0011 secretome

Project description:Macrophage responses to activation are fluid and dynamic in their ability to respond appropriately to challenges, a role integral to host defence. While bacteria can influence macrophage differentiation and polarization into pro-inflammatory and alternatively activated phenotypes through direct interactions, many questions surround indirect communication mechanisms mediated through secretomes derived from gut bacteria, such as lactobacilli. We examined effects of secretome-mediated conditioning on THP-1 human monocytes, focusing on the ability of the Lacticaseibacillus rhamnosus R0011 secretome (LrS) to drive macrophage differentiation and polarization and prime immune responses to subsequent challenge with lipopolysaccharide (LPS). Genome-wide transcriptional profiling revealed increased M2-associated gene transcription in response to LrS conditioning in THP-1 cells. Cytokine and chemokine profiling confirmed these results, indicating increased M2-associated chemokine and cytokine production (IL-1Ra, IL-10). These cells had increased cell-surface marker expression of CD11b, CD86, and CX3CR1, coupled with reduced expression of the M1 macrophage-associated marker CD64. Mitochondrial substrate utilization assays indicated diminished reliance on glycolytic substrates, coupled with increased utilization of citric acid cycle intermediates, characteristics of functional M2 activity. LPS challenge of LrS-conditioned THP-1s revealed heightened responsiveness, indicative of innate immune priming. Resting stage THP-1 macrophages co-conditioned with LrS and retinoic acid also displayed an immunoregulatory phenotype with expression of CD83, CD11c and CD103 and production of regulatory cytokines. Secretome-mediated conditioning of macrophages into an immunoregulatory phenotype is an uncharacterized and potentially important route through which lactic acid bacteria and the gut microbiota may train and shape innate immunity at the gut-mucosal interface.

2024-04-12 | GSE225273 | GEO

Identification of the Senescence Associated Secretory Phenotype from THP-1 Monocytes

Project description:Senescence was induced in THP-1 monocytes in culture and the senescence associated secretory phenotype (SASP) was measured for the complete conditioned medium by applying nanoparticle based Proteograph technology for enrichment followed by DIA mass spectrometry

2024-07-12 | MSV000095315 | MassIVE

Glycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells

Project description:CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-released peptide fractions analyzed by MS/MS. Quantitative assessment revealed differential glycoprotein expression in activated/LPS-tolerized monocytes and naïve monocytes and THP-1 cells.

2017-04-24 | PXD004034 | Pride

A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes.

| S-EPMC11006683 | biostudies-literature

Transcription and chromatin profiling in differentiating THP-1 monocytes and THP-1 derived macrophages

Project description:Here we profile nascent transcription, RNA polymerase III occupancy, chromatin accessibility, and H3K27ac levels in THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to phorbol myristate acetate (PMA).

2017-03-29 | GSE96800 | GEO

Secretome analysis in CAF-dependent conditioned mediums with FaDu cells

Project description:Secretome analysis in CAF-dependent conditioned mediums with FaDu cells

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Proteins secreted by M1 and M2 macrophages derived from human buffy coat or THP-1 cells

Project description:Proteins secreted into the conditioned medium by human monocytic cell line, THP-1, or human buffy coat-derived monocytes polarised to either an M1 (incubation for 48 h with 100 ng/mL LPS and 20 ng/mL IFN-gamma) or M2 (incubation for 48 h with 20 ng/mL IL-4) phenotype. The conditioned medium was enriched for anionic molecules (including proteoglycans) by anion exchange chromatography prior to analysis.

2021-01-21 | MSV000086746 | MassIVE

THP-1 human monocytes Transcriptome following exposure to S-nitrosoglutathione (GSNO)

2015-12-31 | GSE69024 | GEO

THP-1 human monocytes Transcriptome following exposure to S-nitrosoglutathione (GSNO)

Project description:To further study the transcriptome of THP-1 human monocytes after exposure to of S-nitrosoglutathione (GSNO), we investigate whole genome microarray expression to identify genes regulated by exposure to GSNO (1.4 or 6 ÂµM). Changes in gene expression in THP-1 cells incubated without (control) or with (1.4 or 6 ÂµM) of GSNO for 4 h were measured. Four biological replicates were performed as controls: C1; C2; C3; C4. Four biological replicates were performed in 1,4 or 6 ÂµM GSNO-exposed cells: G1; G2; G3; G4 and G5; G6; G7; G8; respectively.

2015-12-31 | E-GEOD-69024 | biostudies-arrayexpress

Differentially expressed genes in pneumococcus-infected THP-1 monocytes

Project description:Differentially expressed genes in pneumococcus-infected THP-1 monocytes

| PRJNA676108 | ENA