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Proteins secreted by M1 and M2 macrophages derived from human buffy coat or THP-1 cells

ABSTRACT: Proteins secreted into the conditioned medium by human monocytic cell line, THP-1, or human buffy coat-derived monocytes polarised to either an M1 (incubation for 48 h with 100 ng/mL LPS and 20 ng/mL IFN-gamma) or M2 (incubation for 48 h with 20 ng/mL IL-4) phenotype. The conditioned medium was enriched for anionic molecules (including proteoglycans) by anion exchange chromatography prior to analysis.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Megan Lord

PROVIDER: MSV000086746 | MassIVE | Thu Jan 21 23:06:00 GMT 2021

REPOSITORIES: MassIVE

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