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Effect of depletion of GPRC5A on gene expression in HT-29 cells upon the treatment of 7-fluorotryptamine and/or TNFa

ABSTRACT: Effect of depletion of GPRC5A on gene expression in HT-29 cells upon the treatment of 7-fluorotryptamine and/or TNFa

PROVIDER: PRJNA935388 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23490100.fastq.gz	Fastqsanger.gz
	SRR23490077.fastq.gz	Fastqsanger.gz
	SRR23490078.fastq.gz	Fastqsanger.gz
	SRR23490079.fastq.gz	Fastqsanger.gz
	SRR23490080.fastq.gz	Fastqsanger.gz

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Effect of depletion of GPRC5A on gene expression in HT-29 cells upon the treatment of 7-fluorotryptamine and/or TNFa

Project description:To investigate the function of GPRC5A and aromatic monoamine, we generated GPRC5A knock-out HT-29 colorectal cancer cells by CRISPR-Cas9 gene editing and evaluated changes in gene expression by RNA sequencing. Wild-type and GPRC5A knock-out cells revealed significant differences in gene expression patterns, particularly the immune- and cancer-related genes. The regulation of gene expression by GPRC5A is not significantly affected by 7-fluorotryptamine and/or TNFa.

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