Project description:This study aimed to evaluate the genomic effects of single nucleotide polymorphisms (SNPs) at the 9p21 locus that are clinically correlated with heart disease. We performed ATAC sequencing on iPSC-derived cardiac fibroblasts and found large differences in AP-1 and GATA family motifs between cells containing the SNPs and those lacking them.
Project description:To examine whether commonly used human cultured fibroblast systems model human cardiac fibroblasts, we tested three different fibroblast cell preparations: primary human cardiac fibroblasts (NHCF), human dermal fibroblasts (NHDF), and immortalized human cardiac fibroblasts (iHCF).
Project description:Normal human cardiac fibroblast were treated with 200 nM halofuginone for 24 hours and the transcriptomic profile was measured by RNAseq.
Project description:The libraries contained in this experiment come from cardiac ventricle fibroblast cells (HCF-av). They are stranded PE101 Illumina Hi-Seq RAMPAGE libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf