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ABSTRACT: Anthocyanins from Malus spp. inhibit the activity of Gymnosporangium yamadae by downregulating the expression of WSC, RLM1, and PMA1

PROVIDER: PRJNA939386 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR23699319_1.fastq.gz	Fastqsanger.gz
	SRR23699319_2.fastq.gz	Fastqsanger.gz
	SRR23699320_1.fastq.gz	Fastqsanger.gz
	SRR23699320_2.fastq.gz	Fastqsanger.gz
	SRR23699321_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose:The red coloration of apple (Malus × domestica Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple.Apple peel can quickly turn red under light conditions after unbagging. Therefore, the implementation of transcriptome sequencing to find genes that promote anthocyanin accumulation in response to light signals is necessary to clarify the mechanism of light-induced anthocyanin accumulation in apple peel.

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Malus

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RNA-SEQ raw data of apple phlomes at bud breaking stage

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Effects of different forms of Anthocyanins on growth inhibition and gene expression of CRC cells

Project description:Scope: Anthocyanins from diet were found to have therapeutic potential for various cancers including colorectal cancer (CRC). Given the diverse forms of anthocyanins, it is unclear whether different types of anthocyanins share common pathways in regulating intestinal cell function. This study aims to investigate the core gene pathways modulated by anthocyanins in intestinal cells, and to identify potential compounds with similar therapeutic effects.Methods and results: Three different forms anthocyanins were used to treat CRC cells at different concentrations and time points, and growth inhibition was observed in all anthocyanins, with varying patterns. RNA-seq analysis showed that the regulatory-pathways of anthocyanins with different structures were different, and the time factor had a greater effect. Time-series analysis of regulatory-genes shared by different concentrations and structures of anthocyanins was performed. By overlapping with public PPAR target gene set, a core gene set across three types of anthocyanins was identified and used in Connectivity Map (CMap) analysis to screen for compounds. The repositioned drug candidates included known anti-cancer drugs that have effects of transcriptome interventions similar to anthocyanins.Conclusion: These findings provide new insights into the mechanisms underlying the anti-CRC effects of anthocyanins and may facilitate the development of novel therapeutic agents for CRC.

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