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Ac4C-RIP-seq to identify mRNA ac4C peaks

ABSTRACT: ac4C-RIP-seq to identify mRNA ac4C peaks

PROVIDER: PRJNA941324 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24593506_1.fastq.gz	Fastqsanger.gz
	SRR24593506_2.fastq.gz	Fastqsanger.gz
	SRR24593507_1.fastq.gz	Fastqsanger.gz
	SRR24593507_2.fastq.gz	Fastqsanger.gz
	SRR24593508_1.fastq.gz	Fastqsanger.gz

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ac4C-RIP-seq to identify mRNA ac4C peaks

Project description:NAT10-catalyzed N4-acetylcytidine (ac4C) has emerged as a vital post-transcriptional modulator on the coding transcriptome by promoting mRNA stability. To explore the transcriptome-wide profile of ac4C modification, we mapped the locations of ac4C modification on wild-type (WT) hESCs and NAT10 KD hESCs by high-throughput ac4C RNA immunoprecipitation sequencing (ac4C-RIP-seq).

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chemical ac4C sequencing (ac4C-seq) to identify mRNA ac4C sites

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chemical ac4C sequencing (ac4C-seq) to identify mRNA ac4C sites

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ac4C RIP-seq analysis of AGS cells upon NAT10 knockout.

Project description:N4-acetylcytidine (ac4C), a conserved chemical modification in eukaryotic prokaryotes that is catalyzed by the N-acetyltransferase 10 (NAT10) enzyme, plays a crucial role in promoting mRNA stability and translation. However, the biological function and mechanisms of NAT10-mediated ac4C in human cancer were poorly defined. In order to investigate the regulatory mechanism of NAT10 in gastric cancer, we performed ac4C RIP-seq(acRIP-seq) analysis in AGS cells with NAT10 knockout compared with control in two repeats.

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Project description:We performedacRIP-seq between control CRC cells and CRC cells with NAT10 knockdown to identify NAT10 mediates mRNA ac4C modification

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Project description:Ac4C-binding RNAs were analyzed by imprinting RNA-sequencing of anti-Ac4C antibody-retrieved complexes from macrophages lysate. We then performed gene expression profiling analysis using data obtained from RIP-seq of peritoneal macrophages incubated with IFN-G

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