Project description:Seperate White and Opaque Candida albicans populations were exposed to a temperature shift from 25 degrees to 37 degrees. Samples were then harvested at various time points to follow the effects of the shift on the two cell types, particularly the opaque cell type which switches to the white cell type after several hours at 37 degrees.
Project description:Prp4-1 and wt strains were grown at 26°C to A600 of 1.0, then an equal volume of 48°C media was added to bring the temperature to 37°C. Both strains were allowed to grow at 37°C and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature. Keywords = splicing Keywords: time-course
Project description:We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. Some are only induced transiently, while are required for long-term adaptation. Many of these genes are related to metabolism, stress response, and immune evasion suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.
Project description:Time-course transcriptional profiling of rice leaf of the temperature shift experiment in a growth chamber. This experiment was performed to validate the results of field transcriptomic modeling. Rice leaves (cv. Norin8) were collected at 2:00 and 14:00 on the day -1, 2, 4 and 6 from the temperature shift (diurnal cycle temperature condition to continuous temperature condition). Two biological replicates for each sampling time point.
