Project description:Histone H2B was mutated to give H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. Experiment Overall Design: mutants compared to wild-type, 3 replicates Experiment Overall Design: This reference Series links data in the following related Series: Experiment Overall Design: GSE3802 Histone H2B^3-32 Experiment Overall Design: GSE3803 Histone H2B K->G Experiment Overall Design: GSE3804 Histone H2B^3-37 Experiment Overall Design: GSE3805 Histone H2B^3-37
Project description:Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates
Project description:Paired-end sequencing study of (1) nucleosome core particles and under-digested chromatin from MNase-treated nuclei; (2) ChIP samples for HA-tagged histone H4 and H2B; (3) ChIP for the Rpb3 subunit of Pol II.
Project description:Paired-end sequencing study of (1) nucleosome core particles and under-digested chromatin from MNase-treated nuclei; (2) ChIP samples for HA-tagged histone H4 and H2B; (3) ChIP for the Rpb3 subunit of Pol II. Nucleosomal DNA and immunopurified sonicated DNA fragments were subjected to paired-end sequencing.
