Effect of SlNOR-like1 knockout on gene expression related to cuticle metabolism in tomato exocarp
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ABSTRACT:
PROVIDER: PRJNA945932 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR23901500_1.fastq.gz | Fastqsanger.gz | |||
| SRR23901500_2.fastq.gz | Fastqsanger.gz | |||
| SRR23901501_1.fastq.gz | Fastqsanger.gz | |||
| SRR23901501_2.fastq.gz | Fastqsanger.gz | |||
| SRR23901502_1.fastq.gz | Fastqsanger.gz |
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Project description:To investigate the function of SlNOR-like1 in the regulation of cuticle formation in tomato fruits, we established nor-like1 mutants by CRISPR/Cas9. We then performed gene expression profiling analysis using data obtained from RNA-seq of tomato exocarp of wild type (WT) and nor-like1 mutant at mature green (MG) stage.
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Project description:The leaf miner, Tuta absoluta, is a notorious destructive pest of tomato that causes important losses worldwide. Breeding of resistant/tolerant tomato cultivars could be an effective strategy for T. absoluta management but, despite the economic importance of tomato, very limited information is available about its response to this treat. To elucidate the defense mechanisms to the herbivore feeding a comparative analysis was performed between a tolerant and susceptible cultivated tomato at both morphological and at transcriptome level to highlight constitutive leaf barriers, molecular and biochemical mechanisms to counter the effect of T. absoluta attack. The tolerant genotype showed an enhanced constitutive barrier due to the higher density of trichomes and increased inducible reactions upon mild infestation thanks to the activation/repression of key transcription factors regulating a higher number of genes involved in cuticle formation and cell wall strength as well as of antinutritive enzymes, and genes involved in the production of chemical toxins and bioactive secondary metabolites. Overall, our findings provide an important foundation for further understanding the insect resistance mechanisms of resistant/tolerant varieties that can be exploited for developing T. absoluta tolerant cultivars, acting as important component of integrated pest management strategy for more sustainable production.
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Project description:The cuticles of arthropods, including aquatic crustaceans like Daphnia, provide an interface between the organism and its environment. Thus, the cuticle’s structure influences how the organism responds to and interacts with its surroundings. Here, we used label-free quantification proteomics to provide a proteome of the molted cuticle of Daphnia magna, which has long been a prominent subject of studies on ecology, evolution, and developmental biology, anddetected 278 high confidence proteins. Using protein sequence domain and functional enrichment analyses, we identified chitin-binding structural proteins and chitin modifying enzymes as most abundant protein groups in the cuticle proteome.Structural cuticular protein families showed a similar distribution to those found in other arthropods and indicated proteins responsible for the soft and flexible structure of the Daphnia cuticle . Finally, cuticle protein genes were clustered as tandem gene arrays in the Daphnia genome, indicating their importance for adaptation to environmental change. The cuticle proteome presented here will be a valuable resource to the Daphnia research community, informing investigations on diverse topics such as the genetic basis of interactions with predators and parasites.
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