Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells [scRNA-seq]

ABSTRACT: Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells [scRNA-seq]

PROVIDER: PRJNA946725 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Comparative gene expression analysis of the human periodontal ligament and dental pulp in the human permanent teeth

Project description:The periodontal ligament(PDL) and dental pulp tissues of human permanent teeth have a number of differences in their developmental processes, histological characteristics and functions. It can be figured out that these differences are attributable to genetic backgrounds of their cells organized tissues. The purpose of this study was to identify the gene-expression profiles and their molecular biological differences of periodontal ligament and dental pulp tissues from the human permanent teeth.

2016-11-22 | GSE50639 | GEO

Proteomic profile of human stem cells from dental pulp and periodontal ligament

Project description:Dental stem cells isolated from oral tissues have been shown to provide with high proliferation ability and multi-lineage differentiation potential. Although the dental stem cells possess the potential of nerve regeneration, the specific expression profile of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) are still unclear. Here, DPSCs and PDLSCs, collected from the same tooth with 3 donors, were tested for high-throughput protein profiles by combining two-dimensional gel proteomics and TMT-based proteomics.

2021-06-10 | PXD021997 | Pride

Comparative gene expression analysis of dental follicle and periodontal ligament in humans

Project description:The aim of this study was to evaluate and compare the gene expression profiles of dental follicle and periodontal ligament in humans, which can possibly explain their functions of dental follicle and PDL such as eruption coordination and stress resorption. That may apply this information to clinical problem like eruption disturbance and to periodontal tissue engineering. PDL samples were obtained from permanent premolars (n=11) and dental follicle samples were obtained during extraction of supernumerary teeth (n=4). Comparative cDNA microarray analysis revealed several differences in gene expression between permanent PDL and dental follicles.

2014-08-21 | E-GEOD-51342 | biostudies-arrayexpress

Comparative gene expression analysis of dental follicle and periodontal ligament in humans

2014-08-21 | GSE51342 | GEO

Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells [scRNA-seq]

Project description:In this study, we investigated the heterogeneity of DPSC and PDLSC cells using scRNA-seq and characterized the molecular features of each cell group. Combining the analyses of trajectory route and scoring, our results demonstrated that DPSCs displayed higher differentiation potentials, while PDLSCs exhibited more matured features. These findings provide a new insight into the complex landscape and functions of oral stem cells, which would enrich our understaning on their roles in embryonic development and regeneration of lost tissues due to dental and periodontal infections.

2023-12-11 | GSE227731 | GEO

Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells

Project description:Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells

| PRJNA946721 | ENA

Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells [BulkRNA-seq]

Project description:Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells [BulkRNA-seq]

| PRJNA946724 | ENA

Consequences of decreased TNAP activity during osteogenic differentiation of periodontal ligament cells – a new model for dental defects in hypophosphatasia

Project description:The effects of TNAP inhibition with levamisole during osteogenic differentiation were analyzed in primary human periodontal ligament cells since patients suffering from hypophosphatasia often display dental symptoms.

2020-04-30 | GSE131338 | GEO

miRNA-720 regulates stem cell phenotype, proliferation and differentiation of dental pulp cells

Project description:Small non-coding microRNAs (miRNAs) are known to play crucial roles in stem cell biology, related to cell reprogramming, maintenance of stemness and regulation of cell differentiation. In an attempt to search for novel miRNAs that can control the fate of dental tissue-derived stem cells, we sorted side population (SP) cells, known to be enriched in stem cells, from dental pulp cells (DPCs) and periodontal ligament cells (PDLCs), and performed a miRNA array. As a result, miR-200b and miR-607 were highly expressed in SP cells, whereas miR-1260b and miR-720 was highly expressed in the differentiated main population (MP) cells. Of note, gain-and-loss of function analysis showed that miR-720 regulates the expression of pluripotency factor NANOG and DNA methyltransferases DNMT3A, DNMT3B and DNMT1 in DPCs. Knockdown of miR-720 significantly increased the levels of NANOG as well as the number of cells positive to the stem cell marker SSEA-4, but down-regulated the levels of DNMTs. miR-720 also regulated the proliferation of DPCs as determined by immunocytochemical analysis against ki-67, as well as odontogenic differentiation demonstrated by alizarin red staining, alkaline phosphatase activity and osteopontin mRNA level. Our findings identify mi-720 as a novel miRNA involved in the regulation of stem cell fate of DPCs. Two cell types (dental pulp cells(DPCs), periodontal ligament cells(PDLCs)) were sorted by FACS to isolate side population (SP) and main population (MP) cells. Then a microRNA array was performed with the SP and MP cells of DPCs and PDLCs.

2013-05-17 | E-GEOD-47025 | biostudies-arrayexpress

scRNA-seq analysis of murine periodontal ligament

Project description:scRNA-seq analysis of murine periodontal ligament

| PRJNA812401 | ENA