Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

THP1 cell response to Mycobacterium tuberculosis infection and Mtb PAMP stimulation

ABSTRACT: THP1 cell response to Mycobacterium tuberculosis infection and Mtb PAMP stimulation

PROVIDER: PRJNA947181 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

THP1 cell response to Mycobacterium tuberculosis infection and Mtb PAMP stimulation

Project description:To compare gene expression changes induced by infection with Mycobacterium tuberculosis (Mtb) with changes induced by purified Mtb products, we infected THP-1 cells with Mtb strain H37Rv or treated with purified Mtb products, then performed RNAseq.

2023-05-15 | GSE227851 | GEO

Genome-wide analysis of Mtb-infected THP1 cells in response to resveratrol treatment

Project description:Analysis of resveratrol-treated cells at gene expression level. The hypothesis tested in the present study was that resveratrol can improve Mycobacterium tuberculosis (Mtb) treatment. Results provide important information of the host response to Mtb infection when given resveratrol, such as resveratrol-mediated modulation of inflammatory pathways induced upon infection.

2017-02-23 | GSE78233 | GEO

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of human THP1 cells

Project description:Mycobacterium tuberculosis (Mtb) is well adapted to survive in macrophages and usually subverts the bactericidal mechanisms of these professional phagocytes. The adaptation of Mtb to the intracellular life depends on its ability to regulate the expression of its genes. Among the most important bacterial transcription activators are the sigma factors that bind to the RNA polymerase and give it promotor specificity. Sigma factor E (SigE) controls the expression of genes that are essential for Mtb virulence. Analysis of the macrophage transcriptional response indicated that proteins encoded by the sigE regulon are involved in the modulation of the macrophage inflammatory response. We compared the global gene expression of THP1 macrophages infected with H37Rv and SigE to the gene expression profile of uninfected macrophages.

2010-05-25 | E-GEOD-7870 | biostudies-arrayexpress

RNA-seq of Control & NCoR1 knockdown THP1 cells infected with Mycobacterium tuberculosis (H37Rv)

Project description:Control and NCoR1 knockdown THP1 cells infected with Mycobacterium tuberculosis (H37Rv) and RNA sequencing were performed in four different conditions (uninfected, 0hr, 12hr, 24hr) including timepoints. The sequencing libraries were prepared using TruSeq Stranded Total RNA Library Prep Kit and sequenced in NextSeq 550.

2023-07-01 | E-MTAB-11499 | biostudies-arrayexpress

Macrophage proteome in response to Mycobacterium tuberculosis infection

Project description:This study investigates the proteomic alterations in human monocytic cell line (THP1) subsequent to infection with different strains of mycobacterium tuberculosis (H37Ra, H37Rv, BND433 and JAL2287) using the SWATH-MS strategy. THP1 proteome was analysed after 6, 18, 30 and 42 hours of infection with each strains and compared to that of uninfected cells. Our findings reveal temporal regulation of host response subsequent to Mtb infection.

2021-01-05 | PXD022352 | Pride

Macrophage proteome in response to Mycobacterium tuberculosis infection

2020-09-23 | PXD009046 | Pride

RNAseq data from intravascular vs parenchymal cells during Mtb infection

Project description:Bulk RNAseq data looking at transcriptional differences between Mtb-specific T cells in the lung, vs those in the vasculature (with naive, CD44low controls) during Mycobacterium tuberculosis infection. Allowing us an unbiased way to discern differentially expressed genes in the two tissue spaces.

2020-12-29 | GSE134186 | GEO

The effect of Mtb aggregation on monocyte-derived macrophage transcription profiles

Project description:Mycobacterium tuberculosis (Mtb) bacilli readily aggregate. We previously reported that Mtb aggregates lead to phagocyte death and subsequent efficient replication in the dead infected cells. Here we examined the transcriptional response of human monocyte derived macrophages to phagocytosis of aggregated Mtb relative to phagocytosis of non-aggregated single or multiple bacilli. Infection with aggregated Mtb led to an early upregulation of pro-inflammatory associated genes and enhanced TNF-alpha signaling via the NFkappaB pathway. These pathways were significantly more highly upregulated relative to infection with single or multiple non-aggregated bacilli per cell.

2021-04-30 | GSE173560 | GEO

System-wide identification of myeloid markers of TB disease and HIV-induced reactivation in the macaque model of Mtb infection and Mtb/SIV co-infection

Project description:Mycobacterium tuberculosis (Mtb) has developed specialized mechanisms to parasitize its host cell, the macrophage. These mechanisms allow it to overcome killing by oxidative burst and persist in the wake of an inflammatory response. Mtb infection in the majority of those exposed is controlled in an asymptomatic form referred to as latent tuberculosis infection (LTBI). HIV is a well-known catalyst of reactivation of LTBI to active TB infection (ATB). Through the use of nonhuman primates (NHPs) co-infected with Mtb and Simian Immunodeficiency Virus (Mtb/SIV), we are able to simulate human progression of TB/AIDS comorbidity. The advantage of NHP models is that they recapitulate the breadth of human TB outcomes, including immune control of infection, and loss of this control due to SIV co-infection. Using macaques infected with Mtb or Mtb/SIV and with different clinical outcomes we attempted to identify signatures between those that progress to active infection after SIV challenge (reactivators) and those that control the infection (non-reactivators).

2022-09-23 | GSE213850 | GEO

Mycobacterium tuberculosis strain:Mtb

Project description:Mycobacterium tuberculosis strain:Mtb Genome sequencing and assembly

| PRJNA638161 | ENA