Dataset Information

Macrophage proteome in response to Mycobacterium tuberculosis infection

ABSTRACT: This study investigates the proteomic alterations in human monocytic cell line (THP1) subsequent to infection with different strains of mycobacterium tuberculosis (H37Ra, H37Rv, BND433 and JAL2287) using the SWATH-MS strategy. THP1 proteome was analysed after 6, 18, 30 and 42 hours of infection with each strains and compared to that of uninfected cells. Our findings reveal temporal regulation of host response subsequent to Mtb infection.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human) Mycobacterium Tuberculosis

TISSUE(S): Macrophage

SUBMITTER: Ajay Kumar

LAB HEAD: Ajay Kumar

PROVIDER: PXD022352 | Pride | 2021-01-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AJAYTHP1_WHOLECELLLIBRARY_UNINFECTED_80mM_F3.wiff	Wiff
	AJAYTHP1_WHOLECELLLIBRARY_UNINFECTED_80mM_F3.wiff.scan	Wiff
	AJAY_THP1_LIBRARY_Long_Column_whole_cell_species_none_WCNT.group	Other
	AJAY_THP1_LIBRARY_Long_Column_whole_cell_species_none_WCNT__FDR.xlsx	Xlsx
	AJAY_THP1_WHOLE_CELL_BND_18HRS_SWATH1.wiff	Wiff

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Publications

THP1 proteomics in response to <i>mycobacterium tuberculosis</i> infection.

Kumar Ajay A Midha Mukul K MK Rao Kanury Vs KV

Data in brief 20210130

Temporal data on how the mycobacterium infection establishes itself inside the host cell is not available. We differentiated human THP1 cell line with PMA and infected them with different laboratory (H37Ra and H37Rv) and clinical strains (BND433 and JAL2287) of mycobacterium tuberculosis (Mtb). Uninfected differentiated THP1 cells were used as infection control. Host proteome was investigated at four different time points to understand the dynamics of host response to mycobacterial infection wit ...[more]

PMID: 33659582

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