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Allometric tissue-scale forces activate mechanoresponsive immune cells to determine pathological foreign body response to biomedical implants

ABSTRACT: Allometric tissue-scale forces activate mechanoresponsive immune cells to determine pathological foreign body response to biomedical implants

PROVIDER: PRJNA947267 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23934576.fastq.gz	Fastqsanger.gz
	SRR23934577.fastq.gz	Fastqsanger.gz
	SRR23934578.fastq.gz	Fastqsanger.gz
	SRR23934579.fastq.gz	Fastqsanger.gz

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Allometric tissue-scale forces activate mechanoresponsive immune cells to determine pathological foreign body response to biomedical implants

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Project description:This study dermined the temporal gene expression profile of myeloid subpopulations recruited to the peritoneal cavity to encapsulate implanted foreign material. Sterile foreign objects were inserted into the peritoneal cavities of MacGreen mice (in which the Csf1r promoter directs myeloid-specific EGFP expression). At various time-points post-surgery (days 2, 4, 7, 14), mice were euthanased, and peritoneal exudate cells removed by lavage. Peritoneal exudate cells from MacGreen mice that had not received implants were used as controls (day 0; 'unstimulated'). Free-floating objects encapsulated with tissue were removed from the peritoneal cavities of different mice at days 7, 14, 21, 28 days, and single cell suspensions obtained by collagenase digestion. Single-cell suspensions from peritoneal exudate or tissue capsules were separated on the basis of size/granularity and EGFP fluorescence using FacsVantage SE Diva. Total RNA was extracted from FACS-sorted EGFP-hi cells.

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