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Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres

ABSTRACT: Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres

PROVIDER: PRJNA947970 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23949300.fastq.gz	Fastqsanger.gz
	SRR23949301.fastq.gz	Fastqsanger.gz
	SRR23949302.fastq.gz	Fastqsanger.gz
	SRR23949303.fastq.gz	Fastqsanger.gz
	SRR23949304.fastq.gz	Fastqsanger.gz

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Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres

Project description:Previously, we reported upregulation of cardiac proliferation in cardiac progenitors derived from human induced pluripotent stem cells (hiPSC-CPCs) that were exposed to space microgravity for 3 days. The overall objective of this investigation was to understand how long-term exposure to microgravity affects the expansion and differentiation of hiPSC-CPCs. Cryopreserved cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) were exposed to space microgravity for 3 weeks in the International Space Station (ISS) and retrieved for transcriptomic profiling. RNA-seq analysis and gene ontology analysis revealed upregulation of cardiac tissue function and morphological terms while downregulating inflammation and ECM regulation terms. Upregulation of genes associated with cell cycle regulation and proliferation were notable as well. Combined with previous work, these results suggest that space microgravity improved cardiac differentiation and structure formation and potentially, proliferation.

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Microgravity on the International Space Station promotes survival and proliferation of human stem cell cardiomyocytes

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Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres.

Project description:Efficient generation of cardiomyocytes from human-induced pluripotent stem cells (hiPSCs) is important for their application in basic and translational studies. Space microgravity can significantly change cell activities and function. Previously, we reported upregulation of genes associated with cardiac proliferation in cardiac progenitors derived from hiPSCs that were exposed to space microgravity for 3 days. Here we investigated the effect of long-term exposure of hiPSC-cardiac progenitors to space microgravity on global gene expression. Cryopreserved 3D hiPSC-cardiac progenitors were sent to the International Space Station (ISS) and cultured for 3 weeks under ISS microgravity and ISS 1 G conditions. RNA-sequencing analyses revealed upregulation of genes associated with cardiac differentiation, proliferation, and cardiac structure/function and downregulation of genes associated with extracellular matrix regulation in the ISS microgravity cultures compared with the ISS 1 G cultures. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes mapping identified the upregulation of biological processes, molecular function, cellular components, and pathways associated with cell cycle, cardiac differentiation, and cardiac function. Taking together, these results suggest that space microgravity has a beneficial effect on the differentiation and growth of cardiac progenitors.

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