Ontology highlight
ABSTRACT:
PROVIDER: PRJNA950209 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Dissimilatory iron reduction by hyperthermophilic archaea occurs in many geothermal environments and generally relies on microbe-mineral interactions that transform various iron oxide minerals. In this study, the physiology of dissimilatory iron and nitrate reduction was examined in the hyperthermophilic crenarchaeon Pyrodictium delaneyi Su06T. Protein electrophoresis showed that the c-type cytochrome and general protein compositions of P. delaneyi changed when grown on ferrihydrite relative to nitrate. Differential proteomic analyses using tandem mass tagged protein fragments and mass spectrometry detected 660 proteins and differential production of 127 proteins. Among these, two putative membrane-bound molybdopterin-dependent oxidoreductase complexes increased in relative abundance 60- to 3,000-fold and 50-100-fold in cells grown on iron oxide. A putative 8-heme c-type cytochrome was 60-fold more abundant in iron grown cells and was unique to the Pyrodictiaceae. There was also a >14,700-fold increase in a membrane transport protein in iron grown cells. There were no changes in the abundances of flagellin proteins nor a putative nitrate reductase, but a membrane nitric oxide reductase was more abundant on nitrate. These data help to elucidate the mechanisms by which hyperthermophilic crenarchaea generate energy and transfer electrons across the membrane to iron oxide minerals.
2020-12-23 | PXD020757 | Pride
Project description:Dissimilatory nitrate reduction to ammonia (DNRA) is an important part of the microbial N-cycle both in natural and man-made habitats, although its significance in wastewater treatment plants is not well understood. Nitrate-limited enrichments from activated sludge with acetate as e-donor consistently resulted in a domination of two closely related genotypes of ammonifying members of Deltaproteobacteria belonging to the genus Geobacter. One of the two was isolated in pure culture which appears to be an extremely specialized ammonifier, actively growing only with acetate as e-donor and C source and nitrate as e-acceptor. The shotgun proteomic raw data obtained from whole cell lysates are provided. First and corresponding author: Dimitry Y. Sorokin soroc@inmi.ru; d.sorokin@tudelft.nl
2023-07-11 | PXD031212 | Pride
Project description:Reforestation is effective in restoring ecosystem functions and enhancing ecosystem services of degraded land. The three most commonly employed reforestation methods of natural reforestation, artificial reforestation with native Masson pine (Pinus massoniana Lamb.), and introduced slash pine (Pinus elliottii Engelm.) plantations were equally successful in biomass yield in southern China. However, it is not known if soil ecosystem functions, such as nitrogen (N) cycling, are also successfully restored. Here, we employed a functional microarray to illustrate soil N cycling. The composition and interactions of N-cycling genes in soils varied significantly with reforestation method. Natural reforestation had more superior organization of N-cycling genes, and higher functional potential (abundance of ammonification, denitrification, assimilatory, and dissimilatory nitrate reduction to ammonium genes) in soils, providing molecular insight into the effects of reforestation.
2019-11-04 | GSE100379 | GEO
Project description:Acididesulfobacillus acetoxydans is an acidophilic sulfate reducer that can dissimilatory reduce nitrate to ammonia (DNRA). However, no known nitrite reductase is encoded. This study was performed to investigate how A. acetoxydans reduces nitrate to nitrite and elucidated a novel DNRA mechanism and potential nitrosative stress resistance mechanisms in acidophiles.
2024-01-26 | PXD045135 | Pride
Project description:PAH degradation under dissimilatory nitrate reducing conditions
| PRJNA374525 | ENA
Project description:The role of sulfur cycle and enzyme activity in dissimilatory nitrate reduction processes in heterotrophic sediments
| PRJNA784358 | ENA
Project description:The role of sulfur cycle and enzyme activity in dissimilatory nitrate reduction processes in heterotrophic sediments
| PRJNA784359 | ENA