Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq of RANKL treated mouse intestinal organoids

ABSTRACT: Single-cell RNA-seq of RANKL treated mouse intestinal organoids

PROVIDER: PRJNA950457 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell RNA-seq of RANKL treated mouse intestinal organoids

Project description:We generated intestinal organoids from a C57BL6/J mouse and stimulated them with mouse recombinant RANKL. We dissociated them into single cells and generated single-cell RNA-seq data from them.

2024-09-07 | GSE228620 | GEO

RNA-seq of RANKL treated mouse intestinal organoids

Project description:RNA-seq of RANKL treated mouse intestinal organoids

| PRJNA936084 | ENA

RNA-seq of RANKL treated mouse intestinal organoids

Project description:We generated intestinal organoids from a C57BL6/J mouse and stimulated them with mouse recombinant RANKL. We generated bulk RNA-seq data from them.

2024-09-07 | GSE225511 | GEO

single cell RNA sequencing for the paired TNF-free and -treated intestinal organoids

Project description:Organoids grown from intestinal crypts of controls and patients with Crohn's disease were sub-cultured at least 6 passages. Single cell RNA sequencing was performed for the paired TNF-free and -treated control and Crohn's organoids.

2020-07-05 | GSE153802 | GEO

Gdnf-treated murine intestinal organoids and sorted Ret+ and Ret- single cells

Project description:Adjacent stroma, including subepithelial fibroblasts, are believed to coordinate the differentiation process of epithelial cells, but the mechanisms are not well understood. Glial cell line-derived neurotrophic factor (GDNF) is expressed in the intestinal Pdgfra high subepithelial myofibroblasts (SEMFs), while the GDNF receptor RET is expressed in a subset of enteroendocrine cells (EECs), indicating regulatory crosstalk. In this experiment, single RET+ intestinal cells were sorted the gene expression profile was compared to non-RET cells. This provided knowledge about RET+ cell types. RET+ cells were upregulated with most of the EEC markers, indicating that RET+ cells are expressed in EECs. We also observed the differences in the gene expression profile of stromal-derived GDNF ligand on intestinal organoids. Organoids were derived from intestinal crypts of WT mice C57BL/6J and treated with 500ng/ml of Gdnf and Gfra1. GDNF-treated organoids induced the expression of EEC genes such as Pyy, Tac1, Tph1, and Cck, indicating enhanced differentiation of EC cell and L-I-N cell lineages. This highlights a stroma-epithelium crosstalk pathway regulating the differentiation of intestinal EEC subtypes.

2024-10-17 | GSE259326 | GEO

Gene expression profile of murine intestinal epithelial cells after RANKL treatment

Project description:RANKL (receptor acrivator of NFkB ligand) is a member of TNF superfamily cytokines. In the gastrointestinal tract, RANKL is expressed in the stromal cells of Peyer's patches, and involved in the development of the specialized intestinal epithelial cells, called M cells. To identify the genes involved in M-cell development, we treated BALB/c mice with recombinant GST-RANKL. After RANKL-treatment, epithelial cells were isolated from small intestine, and used for microarray analysis. Total of 15 samples were analyzed. We generated the Excel sheet for comparing gene expression.

2012-06-16 | E-GEOD-37861 | biostudies-arrayexpress

Gene expression profile of murine intestinal epithelial cells after RANKL treatment

2012-06-17 | GSE37861 | GEO

RNA-seq of mouse small intestinal organoids treated with interferon-gamma

Project description:RNA-seq of C57BL/6 derived small intestinal organoids treated with interferon-gamma. Control group represents non-treated group.

2021-01-21 | E-MTAB-9756 | biostudies-arrayexpress

RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

Project description:To assess the role of LSD1 in mice small intestinal epithelium, small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, paneth cells dissappear upon GSK-LSD1 treatment. We used these sequencing data to show that these small intestinal organoids have a similar phenotype as mice epithelium without LSD1.

2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress

Untargeted metabolomics of intestinal organoids treated with fructose

Project description:We performed untargeted metabolomics of intestinal organoids treated with fructose (10 mM) for 24 hr.At the 5th day of secondary organoid culture, the organoids were treated with fructose (10 mM D-fructose)-containing IntestiCult organoid growth medium for 24 hr and washed with pre-cooled PBS before harvesting.

2020-12-09 | ST001621 | MetabolomicsWorkbench

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data